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AB208003

Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free

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(9 Publications)

Rabbit Recombinant Monoclonal Caspase-3 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 9 publications.

View Alternative Names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (AB208003)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (AB208003)

ab32042, at dilution of 1/100, staining HeLa cells by Immunofluorescence.
Left image : control.
Right image : staurosporine treated. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32042).

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (AB208003)
  • WB

Supplier Data

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (AB208003)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32042). Anti-CASP3 antibody [E83-77] (ab32042) staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32042 was shown to bind specifically to CASP3. A band was observed at 16/28 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in CASP3 knockout cell line. To generate this image, wild-type and CASP3 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (<a href='/en-us/products/primary-antibodies/cleaved-caspase-3-antibody-e83-77-ab32042'>ab32042</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa Treated Staurosporine (2uM, 4h) cell lysate at 20 µg

Lane 2:

CASP3 knockout HeLa Treated Staurosporine (2uM, 4h) cell lysate at 20 µg

Lane 3:

Wild-type HeLa Vehicle Control Staurosporine (0uM, 4h) cell lysate, at 20 µg

Lane 4:

CASP3 knockout HeLa Vehicle Control Staurosporine (0uM, 4h) cell lysate at 20 µg

Predicted band size: 32 kDa

Observed band size: 16 kDa,28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E83-77

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is more sensitive for the detection of cleaved caspase-3 than for pro-caspase-3&#46; For better observation of pro-caspase-3, we recommend ab32150&#46;</p><p>PubMedID 19789217 describes the detection of human cells injected into mice. The pro-caspase-3 is cleaved only when apoptosis event occurs. So, in order to detect active Caspase-3, we strongly suggest to induce your samples into apoptotic pathway.</p>

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Reactivity data

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Product details

ab208003 is the carrier-free version of ab32042.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cleaved Caspase-3 also known as active caspase 3 plays an important role in the execution phase of cell apoptosis. The caspase 3 molecule has a molecular weight of approximately 32 kDa. It undergoes cleavage into subunits that are important for its activation marking its conversion into the cleaved form. Cleaved caspase is widely expressed in various tissues with a notable presence in the cytoplasm of cells undergoing apoptotic processes. Cleavage of caspase 3 results in the formation of a functional enzyme that participates in the degradation of cellular components during apoptosis.
Biological function summary

Caspase 3 acts as an executioner enzyme in the process of programmed cell death. Its cleavage from the inactive zymogen form into the active form triggers apoptotic pathways ensuring the removal of damaged or unnecessary cells. Cleaved caspase operates within a proteolytic cascade often in conjunction with other caspases such as caspase 9. Once activated caspase 3 targets and cleaves specific cellular substrates contributing to the characteristic morphological changes and DNA fragmentation associated with apoptosis.

Pathways

Cleaved caspase 3 is significant within the intrinsic and extrinsic apoptotic pathways. It acts downstream of initiator caspases such as caspase 9 in the intrinsic pathway where its activation involves mitochondrial signals. In the extrinsic pathway it closely associates with caspase 8 mediating cell death signals from extracellular triggers. These pathways ensure that cleaved caspase 3 fulfills its role as a critical mediator of apoptosis linking upstream death signals to cellular dismantling during the apoptosis process.

Cleaved caspase 3 connects to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation of caspase 3 activity may lead to evasion of apoptosis facilitating tumor progression. Therapeutic approaches aim to restore its apoptotic functions in cancer cells. In neurodegenerative diseases like Alzheimer's disease abnormal activation of cleaved caspase 3 contributes to neuronal loss. It interacts with proteins like amyloid precursor protein promoting the pathological changes associated with this disorder. Understanding these interactions provides insights into potential therapeutic avenues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 35338844, PubMed : 35446120, PubMed : 7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed : 18723680, PubMed : 20566630, PubMed : 23650375, PubMed : 7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed : 10497198, PubMed : 16374543, PubMed : 7596430, PubMed : 7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed : 7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed : 37993714, PubMed : 9334240). Involved in the cleavage of huntingtin (PubMed : 8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed : 21357690). Cleaves DSG2 in response to apoptosis resulting in a loss of full length DSG2 at desmosome cell junctions and subsequent loss of cell-cell adhesion (PubMed : 17559062). Also cleaves JUP in response to apoptosis (PubMed : 17559062). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed : 23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed : 30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed : 35338844, PubMed : 35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed : 23845944, PubMed : 33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758104, PubMed : 36758106).
See full target information CASP3
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Pakistan journal of pharmaceutical sciences 38:1632-1646 PubMed40996179

2025

Cantharidic acid inhibits the malignant progression of colorectal cancer by inhibiting aerobic glycolysis and regulating the PI3K/Akt/P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wei,Shulin Dai,Dongyun Zhang,Ting Zhang,Xiaoyu Wang,Bolin Liu,Wei Huang,Yin Li,Mingliao Niu

Scientific reports 14:18586 PubMed39127716

2024

Heterogeneous brain region-specific responses to astrocytic mitochondrial DNA damage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela A Ayala,Anthony Matarazzo,Bonnie L Seaberg,Misha Patel,Eliana Tijerina,Camryn Matthews,Gabriel Bizi,Ashton Brown,Alan Ta,Mendell Rimer,Rahul Srinivasan

Biomedical engineering online 21:53 PubMed35918704

2022

Targeted delivery of irinotecan to colon cancer cells using epidermal growth factor receptor-conjugated liposomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yongwei Liu,Xinghui Li,Renqun Pen,Wei Zuo,Ya Chen,Xiuying Sun,Juhua Gou,Qianwen Guo,Maoling Wen,Wuqi Li,Shuangjiang Yu,Hao Liu,Min Huang

Journal of cellular and molecular medicine 26:3153-3168 PubMed35322540

2022

LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Kang Ho,Danning Zheng,Jiaming Sun,Dongsheng Wen,Shan Wu,Li Yu,Ya Gao,Yifan Zhang,Qingfeng Li

Frontiers in pharmacology 12:783213 PubMed35095494

2022

MicroRNA-92a-3p Enhances Cisplatin Resistance by Regulating Krüppel-Like Factor 4-Mediated Cell Apoptosis and Epithelial-to-Mesenchymal Transition in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Jing Hai,Xuecai Dong,Mengjie Zhang,Shufeng Duan

PLoS genetics 17:e1009834 PubMed34644293

2021

dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yang,Ruiyan Kong,Feng Guang Goh,W Gregory Somers,Gary R Hime,Zhouhua Li,Yu Cai

BioFactors (Oxford, England) 49:79-89 PubMed32048412

2020

Circular RNA-0072309 has antitumor influences in Hep3B cell line by targeting microRNA-665.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyun Yu,Jinhua Dai,Ming Shu

Experimental cell research 383:111505 PubMed31326389

2019

NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jianning Xu,Zhiyong Sheng,Fuxin Li,Shu Wang,Ying Yuan,Meng Wang,Zhihong Yu

Journal of experimental & clinical cancer research 37:157 PubMed30016965

2018

Lycorine inhibits glioblastoma multiforme growth through EGFR suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Shen,Tao Zhang,Zheng Cheng,Ni Zhu,Hua Wang,Li Lin,Zexia Wang,Haotian Yi,Meichun Hu
View all publications
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