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Rabbit Recombinant Monoclonal Lamin-A/C antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free (AB248435), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Predicted
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:2188730, PubMed:22431096, PubMed:2344612, PubMed:23666920, PubMed:24741066, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:24741066, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:23990565, PubMed:25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2188730, PubMed:2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920). Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Alternative names

LMN1, LMNA, Prelamin-A/C

Recommended products

Rabbit Recombinant Monoclonal Lamin-A/C antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4520-11
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248435 is the carrier-free version of Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] ab133269.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cleaved Lamin A + C (Asp230) also known as Progerin refers to a post-translationally modified form of A-type lamins that results from caspase-mediated cleavage at Asp230. The mass of Lamin A is approximately 74 kDa while Lamin C is around 65 kDa. These proteins mainly localize to the nuclear lamina a structural layer beneath the inner nuclear membrane. They express in a variety of tissues but are particularly abundant in cells with a high demand for nuclear structural integrity such as skin heart and muscle tissues.

Biological function summary

These cleaved lamins play a pivotal role in maintaining nuclear shape and stability. They serve as integral components of the nuclear lamina which is part of a complex scaffold that provides mechanical support to the nucleus. Cleaved Lamin A + C also aids in chromatin organization influencing gene expression. Their function impacts key cellular processes like DNA replication and repair as well as regulation of the cell cycle.

Pathways

The involvement of Cleaved Lamin A + C is important within apoptosis and cellular senescence pathways. They interact with proteins such as Caspase-6 and Caspase-7 in these processes. Lamin cleavage leads to nuclear disassembly during programmed cell death while in cellular senescence it signals alterations in nuclear integrity that influence aging-related pathways.

Associated diseases and disorders

Cleaved Lamin A + C associates with accelerated aging disorders such as Hutchinson-Gilford Progeria Syndrome (HGPS). In this condition the accumulation of mutated lamin proteins results in defective nuclear architecture. Disorders like muscular dystrophy present a link as well where abnormal proteins like Emerin and Nesprin could interact with lamins affecting muscle cell viability and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free (ab248435), expandable thumbnail

    Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free (ab248435)

    This data was developed using Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] ab133269, the same antibody clone in a different buffer formulation.

    Note: The observed bands are possible cleavage fragments.

    All lanes: Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] ab133269) at 1/50000 dilution

    Lane 1: Lysate of NIH 3T3 cells treated with staurosporine at 10 µg

    Lane 2: NIH 3T3 cell lysate at 10 µg

    Lane 3: Lysate of HeLa cells treated with camptothecine at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 74 kDa

    Observed band size: 45-50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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