AB256122

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DFNA5/GSDME antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Dfna5, Dfna5h, Gsdme, Gasdermin-E, Non-syndromic hearing impairment protein 5 homolog

1 Images

Supplier Data

Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] - BSA and Azide free (AB256122)

The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

The molecular weight observed is consistent with the literature (PMID : 28459430, 30804337).

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222407).

All lanes:

Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (<a href='/en-us/products/primary-antibodies/cleaved-n-terminal-dfna5-gsdme-antibody-epr20866-160-ab222407'>ab222407</a>) at 1/5000 dilution

Lane 1:

Untreated CT26 (mouse colon carcinoma) whole cell lysate at 20 µg

Lane 2:

CT26 (treated with 2 μg/ml Actinomycin-D for 24 hours) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 35 kDa

false

Exposure time: 103s

Unconjugated

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20866-160

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab256122 is the carrier-free version of ab222407.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cleaved N-terminal DFNA5 also known as GSDME is a significant player in cellular processes. Upon cleaving it turns into an active fragment that carries out distinct functions. GSDME is an effector of necrotic cell death especially pyroptosis with a molecular mass of around 60 kDa. It resides within the cytoplasm of cells displaying expression in various tissues such as the colon stomach and esophagus. This protein functions as a security mechanism modulating cellular responses to stress and damage.

Biological function summary

The cleaved N-terminal fragment of GSDME disrupts the cell membrane using its pore-forming ability. GSDME belongs to the gasdermin family and becomes activated by caspase-3 a well-known executioner of apoptosis. It forms a part of a complex cellular machinery influencing the immune response and cell death. The action of GSDME in the apoptosis or pyroptosis decision is critical since it helps balance cell death pathways and inflammation preventing uncontrolled cell proliferation or excessive inflammation.

Pathways

The cleaved N-terminal GSDME integrates into the intrinsic apoptosis pathway acting downstream of caspase-3 activation. GSDME influences the balance between apoptosis and pyroptosis a critical decision point for cell fate in response to intracellular stress. It works in concert with other gasdermin family proteins such as GSDMD which plays a primary role in pyroptosis. The interaction between these proteins allows cells to select appropriate death pathways under different conditions revealing GSDME’s versatility in cellular functioning.

The function of cleaved N-terminal GSDME links to hearing loss and various cancers. Mutations in GSDME associate with autosomal dominant forms of hearing loss highlighting its role in cochlear function. Furthermore low expression levels of GSDME have connections with cancer cell survival and proliferation. This protein interacts with proteins like caspase-3 in cancer where the cleavage of GSDME may promote pyroptosis over apoptotic pathways offering insights into potential therapeutic targets for tumor management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gasdermin-E. Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. This form constitutes the precursor of the pore-forming protein : upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis.. Gasdermin-E, N-terminal. Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively (PubMed : 32188940). After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis (PubMed : 33852854). Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (By similarity). Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed : 32188940). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed : 28045099). Exhibits bactericidal activity (By similarity). Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity (PubMed : 32188940). Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells : cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression (By similarity). May play a role in the p53/TP53-regulated cellular response to DNA damage (By similarity).

See full target information Gsdme

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:681 PubMed41053117

2025

ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repair.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvia Otchere,Prafulla Shrestha,Himesh N Parmar,Jadyn F Perry,Brittany L Hofmeister,Michelle Steyn,Radhey S Kaushik,Adam D Hoppe,Natalie W Thiex,Ryan L Hanson,Jaime Lopez-Mosqueda,Gergely Imre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] - BSA and Azide free

Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] - BSA and Azide free (AB256122)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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