AB222408

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248]

RabMAb
Recombinant
KO Validated
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(11 Publications)

Rabbit Recombinant Monoclonal DFNA5/GSDME antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

DFNA5, ICERE1, GSDME, Gasdermin-E, Inversely correlated with estrogen receptor expression 1, Non-syndromic hearing impairment protein 5, ICERE-1

1 Images

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Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (AB222408)

Blocking and dilution buffer : 5% NFDM/TBST.

The lysates were kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

The molecular weight observed is consistent with the literature (PMID : 28459430, 30804337).

All lanes:

Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate at 20 µg

Lane 2:

Untreated wild-type SH-SY5Y whole cell lysate at 20 µg

Lane 3:

DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 35 kDa

false

Exposure time: 27s

Carrier free

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20867-248

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody may weakly cross react with the full length DFNA5/GSDME.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Compared with ab222408, <a href='/en-us/products/primary-antibodies/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191'>ab215191</a> has higher sensitivity, we recommend <a href='/en-us/products/primary-antibodies/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191'>ab215191</a> as an alternative for testing N-terminal DFNA5 / GSDME in western blot.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cleaved N-terminal DFNA5 also known as GSDME is a significant player in cellular processes. Upon cleaving it turns into an active fragment that carries out distinct functions. GSDME is an effector of necrotic cell death especially pyroptosis with a molecular mass of around 60 kDa. It resides within the cytoplasm of cells displaying expression in various tissues such as the colon stomach and esophagus. This protein functions as a security mechanism modulating cellular responses to stress and damage.

Biological function summary

The cleaved N-terminal fragment of GSDME disrupts the cell membrane using its pore-forming ability. GSDME belongs to the gasdermin family and becomes activated by caspase-3 a well-known executioner of apoptosis. It forms a part of a complex cellular machinery influencing the immune response and cell death. The action of GSDME in the apoptosis or pyroptosis decision is critical since it helps balance cell death pathways and inflammation preventing uncontrolled cell proliferation or excessive inflammation.

Pathways

The cleaved N-terminal GSDME integrates into the intrinsic apoptosis pathway acting downstream of caspase-3 activation. GSDME influences the balance between apoptosis and pyroptosis a critical decision point for cell fate in response to intracellular stress. It works in concert with other gasdermin family proteins such as GSDMD which plays a primary role in pyroptosis. The interaction between these proteins allows cells to select appropriate death pathways under different conditions revealing GSDME’s versatility in cellular functioning.

The function of cleaved N-terminal GSDME links to hearing loss and various cancers. Mutations in GSDME associate with autosomal dominant forms of hearing loss highlighting its role in cochlear function. Furthermore low expression levels of GSDME have connections with cancer cell survival and proliferation. This protein interacts with proteins like caspase-3 in cancer where the cleavage of GSDME may promote pyroptosis over apoptotic pathways offering insights into potential therapeutic targets for tumor management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gasdermin-E. Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis (PubMed : 27281216, PubMed : 28459430, PubMed : 33852854, PubMed : 35594856, PubMed : 36607699). This form constitutes the precursor of the pore-forming protein : upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed : 28459430).. Gasdermin-E, N-terminal. Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively (PubMed : 27281216, PubMed : 28459430, PubMed : 32188940, PubMed : 33852854, PubMed : 35594856). After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis (PubMed : 28459430, PubMed : 32188940, PubMed : 33852854, PubMed : 35594856). Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed : 28459430). Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed : 27281216, PubMed : 28459430, PubMed : 32188940). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed : 28045099). Exhibits bactericidal activity (PubMed : 27281216). Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity (PubMed : 21522185, PubMed : 32188940). Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells : cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression (PubMed : 31953257, PubMed : 32188940). May play a role in the p53/TP53-regulated cellular response to DNA damage (PubMed : 16897187).. Gasdermin-E, N-terminal. (Microbial infection) Pore-forming protein, which promotes maternal placental pyroptosis in response to Zika virus infection, contributing to adverse fetal outcomes.

See full target information GSDME

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40937577

2025

CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liang,Zhongcheng Zhu,Yangshuo Tang,Shuhua Zhou,Moyan Xiao,Xuejun Gong,Ke Ye

Journal of translational medicine 23:495 PubMed40307853

2025

An optimized integrin α6-targeted peptide capable of delivering toxins for melanoma treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhao,Zi-Qian Li,Ying-Bin Huang,Meng-Meng Liu,Fei Cao,Guo-Long Bu,Peng-Fei Xu,Qi Fang,Zhu-Long Hu,Di Wu,Guo-Kai Feng,Xue-Kui Liu

Scientific reports 15:13280 PubMed40247083

2025

Ocifisertib alleviates the gasdermin D-independent pyroptosis of nucleus pulposus cells by targeting GSDME.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Lin,Qiyong Chen,Shaoqiang Liu,Boling Liu

Research (Washington, D.C.) 8:0594 PubMed39872128

2025

Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Wang,Xunlei Pang,Ruirui Li,Jiuzhou Chen,Chen Wen,Huihuang Zhu,Tingyu Long,Jianjie Li,Lijun Zheng,Youcai Deng,Junnian Zheng,Bo Xu

Cell death discovery 10:227 PubMed38740747

2024

Tumors cells with mismatch repair deficiency induce hyperactivation of pyroptosis resistant to cell membrane damage but are more sensitive to co-treatment of IFN-γ and TNF-α to PANoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyan Li,Hengli Ni,Ying Li,Aijun Zhou,Xiaokang Qin,Yuqing Li,Liheng Che,Hui Mo,Chao Qin,Jianming Li

Gut microbes 16:2333790 PubMed38533566

2024

induces chemoresistance in colorectal cancer by inhibiting pyroptosis via the Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Wang,Lu Zhang,Xiao-Xu Leng,Yi-Le Xie,Zi-Ran Kang,Li-Cong Zhao,Lin-Hong Song,Cheng-Bei Zhou,Jing-Yuan Fang

Cell death & disease 15:163 PubMed38388468

2024

CHOP-mediated Gasdermin E expression promotes pyroptosis, inflammation, and mitochondrial damage in renal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Ma,Hao Lu,Ning Li,Weijian Ni,Wenbo Zhang,Qiang Liu,Wenzheng Wu,Shichao Xia,Jiagen Wen,Tao Zhang

International journal of molecular sciences 24: PubMed37834111

2023

BIRC5 Inhibition Is Associated with Pyroptotic Cell Death via Caspase3-GSDME Pathway in Lung Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingwei Zhang,Ximing Chen,Yingying Hu,Tong Zhou,Menghan Du,Run Xu,Yongchao Chen,Pingping Tang,Zhouxiu Chen,Jiamin Li

Biotechnology journal 18:e2200633 PubMed37204010

2023

Engineering single-domain antibodies targeting Gasdermin E activation by the chemotherapeutic agent cis-diaminodichloroplatinum.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyi Ma,Jintao Xu,Qiuyun Gao,Yanan Sun,Yu Wang,Zhe Liu,Zhenyi Ma

Cell death discovery 8:112 PubMed35279683

2022

HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yao,Zhen Jin,Zihan Zheng,Xiaohan Lv,Lingxuan Ren,Jianjun Yang,Danli Chen,Bo Wang,Wei Yang,Lifang Chen,Weirong Wang,Jianli Gu,Rong Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248]

Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (AB222408)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

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