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AB92373

Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17]

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(1 Publication)

Rabbit Recombinant Monoclonal NEAS antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NEAS, SPTA2, SPTAN1, Alpha-II spectrin, Fodrin alpha chain

1 Images
Western blot - Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (AB92373)
  • WB

Unknown

Western blot - Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (AB92373)

All lanes:

Western blot - Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)

Lane 1:

HeLa + Camptothecin cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat + Staurosporine cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 285 kDa

Observed band size: 150 kDa

false

  • Carrier free

    Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3830-17

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cleaved NEAS (Asp1185) also known as cleaved Notch extracellular truncation and NEAS is a significant enzymatic cleavage product of the Notch receptor involved in signal transduction. It has a mass of about 300 kDa. Cleavage occurs when the receptor undergoes proteolytic processing producing a functional subunit. This subunit is present in various tissues particularly active in developmental tissues and the nervous system. This localization suggests an important role in those areas for proper cell signaling and differentiation processes.
Biological function summary

The Notch intracellular domain (NICD) which arises after further cleavage is a part of the core Notch signaling pathway. Cleaved NEAS is part of a complex involving other cleavage events; it acts as an intermediary in the Notch signaling cascade. This role makes it important for cellular communication and regulation determining cell fate decisions and controlling differentiation and proliferation. Studies often identify its presence and function within the nuclear and membrane-associated areas indicating roles both in direct gene expression and membrane signaling domains.

Pathways

Cleaved NEAS interacts heavily with Delta/Serrate/LAG-2 (DSL) Notch ligands and influences the Notch signaling pathway. The processed NICD translocates to the nucleus affecting the transcription of target genes involved in developmental processes and immune system regulation. In addition to cooperating with ligands such as Jagged and Delta-like proteins NEAS has interactions that play a part in modulating important pathways like Wnt signaling revealing its role in coordinating diverse signaling networks.

Cleaved NEAS’s dysregulation links it to various cancer types including leukemia and breast cancer. Overactive Notch signaling often due to abnormal cleaved NEAS activity can drive oncogenesis and influence tumor progression. NEAS interacts with regulatory proteins like P53 which is also implicated in tumor suppression making it a point of interest in therapeutic strategies. Additionally its association with neurodegenerative diseases such as Alzheimer's disease indicates its relevance in both proliferative and degenerative conditions further highlighting its biological importance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
See full target information SPTAN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mSphere 5: PubMed32024710

2020

Recruitment of Host Nuclear Pore Components to the Vicinity of Schizonts.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Huber,Anina Bär,Selina Epp,Jacqueline Schmuckli-Maurer,Naja Eberhard,Bruno M Humbel,Andrew Hemphill,Kerry Woods
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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