Rabbit Recombinant Monoclonal CLEC1 antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-CLEC1 antibody [EPR27402-62] (AB308635), expandable thumbnail](https://content.abcam.com/products/images/clec1-antibody-epr27402-62-ab308635--western-blot-img445179.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLEC1 antibody [EPR27402-62] (AB308635)")
![Western blot - Anti-CLEC1 antibody [EPR27402-62] (AB308635), expandable thumbnail](https://content.abcam.com/products/images/clec1-antibody-epr27402-62-ab308635--western-blot-img409697.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLEC1 antibody [EPR27402-62] (AB308635)")
![Western blot - Anti-CLEC1 antibody [EPR27402-62] (AB308635), expandable thumbnail](https://content.abcam.com/products/images/clec1-antibody-epr27402-62-ab308635--western-blot-img409698.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLEC1 antibody [EPR27402-62] (AB308635)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Alternative names
CLEC1, UNQ569/PRO1131, CLEC1A, C-type lectin domain family 1 member A, C-type lectin-like receptor 1, CLEC-1
Recommended products
Rabbit Recombinant Monoclonal CLEC1 antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27402-62
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635), expandable thumbnail](https://content.abcam.com/products/images/clec1-antibody-epr27402-62-ab308635--western-blot-img445179.jpg "Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635)")
Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.
All lanes: Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635) at 1/1000 dilution
Lane 1: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 2: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 32 kDa
Exposure time: 114s
![Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635), expandable thumbnail](https://content.abcam.com/products/images/clec1-antibody-epr27402-62-ab308635--western-blot-img409697.jpg "Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635)")
Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: kidney (PMID: 29296975), liver (PMID: 10671229, 29296975).
There is an unknown band at 50 kDa.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 32 kDa, 37 kDa
Exposure time: 180s
![Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635), expandable thumbnail](https://content.abcam.com/products/images/clec1-antibody-epr27402-62-ab308635--western-blot-img409698.jpg "Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635)")
Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635)
Blocking and diluting buffer and concentration: 5% NFDM/TBST. CLEC1 is a glycoprotein of approximately 37 kDa and detected as a 32 kDa band after treated with Protein Deglycosylation MIX II.
There is an unknown band at 50kDa.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-CLEC1 antibody [EPR27402-62] (ab308635) at 1/1000 dilution
Lane 1: Untreated human lung tissue lysate at 20 µg
Lane 2: Human lung tissue lysate treated with Protein Deglycosylation Mix II at 25 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 32 kDa, 37 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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