Anti-CLEC1 antibody [EPR27402-62] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-CLEC1 antibody [EPR27402-62] - BSA and Azide free (AB308636)

This data was developed using 308635, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : kidney (PMID : 29296975), liver (PMID : 10671229, 29296975).

There is an unknown band at 50 kDa.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-CLEC1 antibody [EPR27402-62] (<a href='/en-us/products/primary-antibodies/clec1-antibody-epr27402-62-ab308635'>ab308635</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa,37 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CLEC1 antibody [EPR27402-62] - BSA and Azide free (AB308636)

This data was developed using 308635, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST. CLEC1 is a glycoprotein of approximately 37 kDa and detected as a 32 kDa band after treated with Protein Deglycosylation MIX II.  There is an unknown band at 50kDa. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-CLEC1 antibody [EPR27402-62] (<a href='/en-us/products/primary-antibodies/clec1-antibody-epr27402-62-ab308635'>ab308635</a>) at 1/1000 dilution

Lane 1:

Untreated human lung tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate treated with Protein Deglycosylation Mix II at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa,37 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-CLEC1 antibody [EPR27402-62] - BSA and Azide free (AB308636)

This data was developed using ab308635, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

All lanes:

Western blot - Anti-CLEC1 antibody [EPR27402-62] (<a href='/en-us/products/primary-antibodies/clec1-antibody-epr27402-62-ab308635'>ab308635</a>) at 1/1000 dilution

Lane 1:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 32 kDa

false

Exposure time: 114s