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AB315086

Anti-CLEC10A antibody [EPR27400-166]

  • BOND RX™ Validated
  • 20ul selling size
  • Recombinant
  • Advanced Validation
  • RabMAb
  • What is this?

5

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal CLEC10A antibody. Suitable for IHC-P, mIHC and reacts with Human, Transfected cell line - Human samples. Cited in 2 publications.

View Alternative Names

CD301, CLECSF13, CLECSF14, HML, CLEC10A, C-type lectin domain family 10 member A, C-type lectin superfamily member 14, DC-asialoglycoprotein receptor, Macrophage galactose-type lectin, Macrophage lectin 2, DC-ASGPR, MGL

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a CLEC10A expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling CLEC10A with ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Myc-His-tagged CLEC10A construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-CLEC10A antibody [EPR27400-166] (AB315086)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CLEC10A antibody [EPR27400-166] (AB315086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CLEC10A with ab315086 at 1/500 dilution (1.02 μg/mL) (B), Mannose Receptor with ab252921 at 1/2000 dilution (0.502 μg/ml) (C) and CD11c with ab52632 at 1/16000 dilution (0.128 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD11c (magenta; Opal™690), anti-CLEC10A (green; Opal™520) and anti-Mannose Receptor (red; Opal™570) on human colon.
Panel B : anti-CLEC10A stained on dendritic cell subsets.
Panel C : anti-Mannose Receptor stained on dendritic cell subsets.
Panel D : anti-CD11c stained on mainly granulocytes, macrophages, and dendritic cells.

The section was incubated in three rounds of staining : in the order of ab52632, ab315086, and ab252921 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CLEC10A with ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil. The section was incubated with ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLEC10A with ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human colon. The section was incubated with ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] (AB315086)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CLEC10A with ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human prostatic hyperplasia. The section was incubated with ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Carrier free

    Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27400-166

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

mIHC, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

C-type lectin receptor involved in recognition of N-acetylgalactosamine (GalNAc)-terminated glycans by myeloid antigen presenting cells (APCs) (PubMed : 15802303, PubMed : 16998493, PubMed : 17616966, PubMed : 22213806, PubMed : 33724805, PubMed : 8598452). Binds in a Ca(2+)-dependent manner to alpha- and beta-linked GalNAc residues on glycoprotein and glycolipid antigens, including alphaGalNAc- and Galbeta1->3GalNAc-O-Ser/Thr also known as Tn and T antigens, LacdiNAc epitope GalNAcbeta1->4GlcNAc and its derivative GalNAcbeta1->4-(Fucalpha1->3)GlcNAc, O-linked core 5 and 6 glycans, and GM2 and GD2 gangliosides (PubMed : 15802303, PubMed : 23507963). Acts as a signaling receptor at the interface of APC-T cell interactions. On immature dendritic cells, recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and downregulates PTRPN/CD45 phosphatase activity with an impact on T cell activation threshold, cytokine production and proliferation. Modulates dendritic cell maturation toward a tolerogenic phenotype leading to generation of regulatory CD4-positive T cell subset with immune suppressive functions (PubMed : 15802303, PubMed : 16998493, PubMed : 22213806). Acts as an endocytic pattern recognition receptor involved in antitumor immunity. During tumorigenesis, recognizes Tn antigens and its sialylated forms Neu5Ac-Tn and Neu5Gc-Tn expressed on tumor cell mucins. On immature dendritic cells, can internalize Tn-terminated immunogens and target them to endolysosomal compartment for MHC class I and II antigen presentation to CD8-positive and CD4-positive T cells, respectively (PubMed : 15802303, PubMed : 17616966, PubMed : 17804752).
See full target information CLEC10A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113351 PubMed40949104

2025

Decoding functional and developmental trajectories of tissue-resident uterine dendritic cells through integrative omics.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Singh,Jing Yang,Nicholas K Adzibolosu,Songchen Cai,Elana Kauf,Lingtao Yang,Qiyuan Li,Hanjie Li,Alexandra Werner,Siddharth Parthasarathy,Jiahui Ding,Jared Fortier,Marta Rodriguez-Garcia,Lianghui Diao,Gil Mor

Theranostics 14:3526-3547 PubMed38948071

2024

Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma.

Applications

IHC

Species

Human

Yao Li,Fengwei Li,Lei Xu,Xiaodong Shi,Hui Xue,Jianwei Liu,Shilei Bai,Yeye Wu,Zhao Yang,Feng Xue,Yong Xia,Hui Dong,Feng Shen,Kui Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com