Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free

5 product images

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  Multiplex immunohistochemistry - Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free (ab315087)

  This data was developed using Anti-CLEC10A antibody [EPR27400-166] ab315086, the same antibody clone in a different buffer formulation.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CLEC10A with Anti-CLEC10A antibody [EPR27400-166] ab315086 at 1/500 dilution (1.02 µg/mL) (B), Mannose Receptor with Anti-Mannose Receptor antibody [EPR22489-7] ab252921 at 1/2000 dilution (0.502 μg/ml) (C) and CD11c with Anti-CD11c antibody [EP1347Y] - C-terminal ab52632 at 1/16000 dilution (0.128 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-CD11c (magenta; Opal™690), anti-CLEC10A (green; Opal™520) and anti-Mannose Receptor (red; Opal™570) on human colon.

  Panel B: anti-CLEC10A stained on dendritic cell subsets.

  Panel C: anti-Mannose Receptor stained on dendritic cell subsets.

  Panel D: anti-CD11c stained on mainly granulocytes, macrophages, and dendritic cells.

  The section was incubated in three rounds of staining: in the order of Anti-CD11c antibody [EP1347Y] - C-terminal ab52632, Anti-CLEC10A antibody [EPR27400-166] ab315086, and Anti-Mannose Receptor antibody [EPR22489-7] ab252921 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free (ab315087)

  This data was developed using Anti-CLEC10A antibody [EPR27400-166] ab315086, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a CLEC10A expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling CLEC10A with Anti-CLEC10A antibody [EPR27400-166] ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on HEK-293T transfected with a Myc-His-tagged CLEC10A construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with Anti-CLEC10A antibody [EPR27400-166] ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free (ab315087)

  This data was developed using Anti-CLEC10A antibody [EPR27400-166] ab315086, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CLEC10A with Anti-CLEC10A antibody [EPR27400-166] ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on immune cells of human prostatic hyperplasia. The section was incubated with Anti-CLEC10A antibody [EPR27400-166] ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free (ab315087)

  This data was developed using Anti-CLEC10A antibody [EPR27400-166] ab315086, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLEC10A with Anti-CLEC10A antibody [EPR27400-166] ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on immune cells of human colon. The section was incubated with Anti-CLEC10A antibody [EPR27400-166] ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC10A antibody [EPR27400-166] - BSA and Azide free (ab315087)

  This data was developed using Anti-CLEC10A antibody [EPR27400-166] ab315086, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CLEC10A with Anti-CLEC10A antibody [EPR27400-166] ab315086 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human tonsil. The section was incubated with Anti-CLEC10A antibody [EPR27400-166] ab315086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins