Rabbit Recombinant Monoclonal CLEC12A antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (AB307663), expandable thumbnail](https://content.abcam.com/products/images/clec12a-antibody-epr26967-58-bsa-and-azide-free-ab307663--flow-cytometry-img407148.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (AB307663)")
![Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (AB307663), expandable thumbnail](https://content.abcam.com/products/images/clec12a-antibody-epr26967-58-bsa-and-azide-free-ab307663--flow-cytometry-img407149.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (AB307663)")
![Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (AB307663), expandable thumbnail](https://content.abcam.com/products/images/clec12a-antibody-epr26967-58-bsa-and-azide-free-ab307663--flow-cytometry-img407150.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (AB307663)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation (PubMed:14739280, PubMed:15238421, PubMed:16239426, PubMed:34234773, PubMed:38367667, PubMed:38386511, PubMed:39143217). Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation (By similarity). Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals (PubMed:38367667, PubMed:38386511, PubMed:39143217). Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid (PubMed:31269448, PubMed:36542980). Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation (PubMed:14739280, PubMed:16239426, PubMed:34234773). Acts as a pattern-recognition receptor for NETs in neutrophils: specifically recognizes DNA in NETs, leading to inhibit neutrophil activation and limit further NET formation (PubMed:39143217). This regulation is essential for controlling key neutrophil responses and limit NET-mediated inflammatory conditions (By similarity). Also recognizes dead cells by acting as a receptor for monosodium urate crystals, leading to down-regulate neutrophil activation (PubMed:38367667, PubMed:38386511). Binding to monosodium urate crystals also promotes the type I interferon response (By similarity). Acts as an inhibitor of natural killer (NK) cell cytotoxicity (PubMed:15238421). Also acts as an ihibitor of dendritic cell maturation in an IL10-dependent manner (PubMed:16239426).
Alternative names
CD371, CLL1, DCAL2, KLRL1, MICL, CLEC12A, C-type lectin domain family 12 member A, C-type lectin-like molecule 1, Dendritic cell-associated lectin 2, Killer cell C-type lectin-like receptor L1, Myeloid inhibitory C-type lectin-like receptor, CLL-1, DCAL-2, hKLRL1
Recommended products
Rabbit Recombinant Monoclonal CLEC12A antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26967-58
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663), expandable thumbnail](https://content.abcam.com/products/images/clec12a-antibody-epr26967-58-bsa-and-azide-free-ab307663--flow-cytometry-img407148.jpg "Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663)")
Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663)
This data was developed using Anti-CLEC12A antibody [EPR26967-58] ab307662, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / U-937 (human histiocytic lymphoma monocyte, Right) cells labelling CLEC12A with Anti-CLEC12A antibody [EPR26967-58] ab307662 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.![Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663), expandable thumbnail](https://content.abcam.com/products/images/clec12a-antibody-epr26967-58-bsa-and-azide-free-ab307663--flow-cytometry-img407149.jpg "Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663)")
Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663)
This data was developed using Anti-CLEC12A antibody [EPR26967-58] ab307662, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CLEC12A with Anti-CLEC12A antibody [EPR26967-58] ab307662 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-CLEC12A antibody [EPR26967-58] ab307662. Then stained with anti-CD14 conjugated to Alexa Fluor® 647.Gated on viable cells.![Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663), expandable thumbnail](https://content.abcam.com/products/images/clec12a-antibody-epr26967-58-bsa-and-azide-free-ab307663--flow-cytometry-img407150.jpg "Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663)")
Flow Cytometry - Anti-CLEC12A antibody [EPR26967-58] - BSA and Azide free (ab307663)
This data was developed using Anti-CLEC12A antibody [EPR26967-58] ab307662, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CLEC12A with Anti-CLEC12A antibody [EPR26967-58] ab307662 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-CLEC12A antibody [EPR26967-58] ab307662. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.Gated on viable cells.
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