AB239222

Anti-CLEC2D antibody

Name: Anti-CLEC2D antibody (ab239222) | Abcam
Brand: Abcam Limited
SKU: AB239222
Price: 525 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal CLEC2D antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CLEC2D aa 100 to C-terminus.

View Alternative Names

CLAX, LLT1, OCIL, CLEC2D, C-type lectin domain family 2 member D, Lectin-like NK cell receptor, Lectin-like transcript 1, Osteoclast inhibitory lectin, LLT-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CLEC2D antibody (AB239222)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLEC2D antibody (AB239222)

Paraformaldehyde fixed A431 (Human epidermoid carcinoma cell line) cells, permeabilized with 0.15% Triton, stained for CLEC2D (green) using ab239222 at 10 μg/ml in ICC/IF. Primary incubation for 1 hour followed by Alexa Fluor^®488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue).

Negative control : Non-immunized goat IgG (10 μg/ml) followed by Alexa Fluor^®488 secondary antibody (2 μg/ml).

Flow Cytometry (Intracellular) - Anti-CLEC2D antibody (AB239222)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CLEC2D antibody (AB239222)

Flow cytometric analysis of paraformaldehyde fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells (blue line), permeabilized with 0.5% Triton. Primary incubation time 1 hour (10 μg/ml) followed by an Alexa-Fluor^®488 secondary antibody (1 μg/ml).

IgG control : Non-immunized goat IgG (black line) followed by an Alexa-Fluor^®488 secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CLEC2D aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UHP7

Specificity

This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis (PubMed : 16339513, PubMed : 20843815). Inhibits osteoclast formation (PubMed : 14753741, PubMed : 15123656). Inhibits bone resorption (PubMed : 14753741). Modulates the release of interferon-gamma (PubMed : 15104121). Binds high molecular weight sulfated glycosaminoglycans (PubMed : 15123656).

See full target information CLEC2D

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 18:1552819 PubMed40206188

2025

The restoration of REST inhibits reactivity of Down syndrome iPSC-derived astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Huang,Sharida Fakurazi,Pike-See Cheah,King-Hwa Ling

Heliyon 10:e27354 PubMed38509909

2024

C-type lectin 2D (CLEC2D) is upregulated in clear cell renal cell carcinoma (ccRCC) tissues and predicts poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huibing Li,Pengyi Zheng,Zhijun Li,Qingjiang Han,Bisheng Zhou,Kaixuan Wang

Shock (Augusta, Ga.) 61:592-600 PubMed37878490

2023

C-TYPE LECTIN-2D RECEPTOR CONTRIBUTES TO HISTONE-INDUCED VASCULAR BARRIER DYSFUNCTION DURING BURN INJURY.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Yang,Ethan Zheng,Xiaoqi Sun,Amanda Reynolds,Monica Gonzalez,Juan Hernandez Villamil,Briana D Pando,David J Smith,Sarah Y Yuan,Mack H Wu

The Prostate 80:742-752 PubMed32449811

2020

Docetaxel suppresses immunotherapy efficacy of natural killer cells toward castration-resistant prostate cancer cells via altering androgen receptor-lectin-like transcript 1 signals.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tang,Shenglin Gao,Lei Zhang,Bianjiang Liu,Jie Li,Zengjun Wang,Wei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLEC2D antibody

Immunocytochemistry/ Immunofluorescence - Anti-CLEC2D antibody (AB239222)

Flow Cytometry (Intracellular) - Anti-CLEC2D antibody (AB239222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise