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AB319140

Anti-CLEC2D antibody [EPR27405-92]

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Rabbit Recombinant Monoclonal CLEC2D antibody. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CLAX, LLT1, OCIL, CLEC2D, C-type lectin domain family 2 member D, Lectin-like NK cell receptor, Lectin-like transcript 1, Osteoclast inhibitory lectin, LLT-1

5 Images
Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)

Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte) (Right) / 293T (human embryonic kidney epithelial cell) (Left) cells labelling CLEC2D with ab319140 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : 293T (PMID : 20843815)

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC2D with ab319140 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD19 conjugated to PE/Cy7.
Gated on viable Lymphocytes.

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC2D with ab319140 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD14 conjugated to FITC.
Gated on viable cells.

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC2D with ab319140 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD56 conjugated to Brilliant Violet 421.
Gated on viable Lymphocytes.

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC2D antibody [EPR27405-92] (AB319140)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling CLEC2D with ab319140 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : Jurkat (PMID : 20843815)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27405-92

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis (PubMed : 16339513, PubMed : 20843815). Inhibits osteoclast formation (PubMed : 14753741, PubMed : 15123656). Inhibits bone resorption (PubMed : 14753741). Modulates the release of interferon-gamma (PubMed : 15104121). Binds high molecular weight sulfated glycosaminoglycans (PubMed : 15123656).
See full target information CLEC2D
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com