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Rabbit Recombinant Monoclonal CLECSF6 antibody. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-CLEC4A antibody [EPR26969-7] (AB308298), expandable thumbnail
  • Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] (AB308298), expandable thumbnail
  • Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] (AB308298), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] (AB308298), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow CytIHC-PIP
Human
Expected
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner (PubMed:27015765). Involved in regulating immune reactivity (PubMed:10438934, PubMed:18258799). Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8(+) T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8(+) T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286). In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799). May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934). (Microbial infection) Involved in the interaction between HIV-1 virus and dendritic cells. Enhances HIV-1 binding/entry and virus infection. Requires ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases and MAP kinases.

Alternative names

CD367, CLECSF6, DCIR, LLIR, HDCGC13P, CLEC4A, C-type lectin domain family 4 member A, C-type lectin DDB27, C-type lectin superfamily member 6, Dendritic cell immunoreceptor, Lectin-like immunoreceptor

Recommended products

Rabbit Recombinant Monoclonal CLECSF6 antibody. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26969-7
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CLEC4A antibody [EPR26969-7] (ab308298), expandable thumbnail

    Western blot - Anti-CLEC4A antibody [EPR26969-7] (ab308298)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human CLEC4C.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-CLEC4A antibody [EPR26969-7] (ab308298) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Human CLEC4A expression vector containi a His-tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a Human CLEC4C expression vector containi a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa

    Observed band size: 35 kDa

    Exposure time: 180s

  • Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] (ab308298), expandable thumbnail

    Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] (ab308298)

    Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with ab308298 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor ® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD3 conjugated to 647. Gated on viable cells.

  • Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] (ab308298), expandable thumbnail

    Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] (ab308298)

    Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with ab308298 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor ® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD11b conjugated to BV421. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] (ab308298), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] (ab308298)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human pbmc (human primary peripheral blood mononuclear cell) cells labelling CLEC4A with ab308298 at 1/50 (10.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in subsets of human PBMCs.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/1000 2 ug/ml dilution.

    Confocal image showing membranous staining in subsets of human PBMCs.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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