Rabbit Recombinant Monoclonal CLECSF6 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.
Images
![Western blot - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--western-blot-img409702.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)")
![Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--flow-cytometry-img409700.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)")
![Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--flow-cytometry-img409699.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)")
![Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--immunocytochemistry-immunofluorescence-img409701.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner (PubMed:27015765). Involved in regulating immune reactivity (PubMed:10438934, PubMed:18258799). Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8(+) T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8(+) T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286). In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799). May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934). (Microbial infection) Involved in the interaction between HIV-1 virus and dendritic cells. Enhances HIV-1 binding/entry and virus infection. Requires ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases and MAP kinases.
Alternative names
CD367, CLECSF6, DCIR, LLIR, HDCGC13P, CLEC4A, C-type lectin domain family 4 member A, C-type lectin DDB27, C-type lectin superfamily member 6, Dendritic cell immunoreceptor, Lectin-like immunoreceptor
Recommended products
Rabbit Recombinant Monoclonal CLECSF6 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26969-7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--western-blot-img409702.jpg "Western blot - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)")
Western blot - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)
This data was developed using 308298, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human CLEC4C.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-CLEC4A antibody [EPR26969-7] (Anti-CLEC4A antibody [EPR26969-7] ab308298) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a Human CLEC4A expression vector containi a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a Human CLEC4C expression vector containi a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa
Exposure time: 180s
![Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--flow-cytometry-img409700.jpg "Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)")
Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)
This data was developed using Anti-CLEC4A antibody [EPR26969-7] ab308298, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with Anti-CLEC4A antibody [EPR26969-7] ab308298 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor ® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD3 conjugated to 647. Gated on viable cells.
![Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--flow-cytometry-img409699.jpg "Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)")
Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)
This data was developed using Anti-CLEC4A antibody [EPR26969-7] ab308298, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with Anti-CLEC4A antibody [EPR26969-7] ab308298 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor ® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD11b conjugated to BV421. Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299), expandable thumbnail](https://content.abcam.com/products/images/clec4a-antibody-epr26969-7-bsa-and-azide-free-ab308299--immunocytochemistry-immunofluorescence-img409701.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)")
Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (ab308299)
This data was developed using Anti-CLEC4A antibody [EPR26969-7] ab308298, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human pbmc (human primary peripheral blood mononuclear cell) cells labelling CLEC4A with Anti-CLEC4A antibody [EPR26969-7] ab308298 at 1/50 (10.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in subsets of human PBMCs.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/1000 2 ug/ml dilution.
Confocal image showing membranous staining in subsets of human PBMCs.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
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