Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)

This data was developed using ab308298, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human pbmc (human primary peripheral blood mononuclear cell) cells labelling CLEC4A with ab308298 at 1/50 (10.06 ug/ml) dilution, followed by ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150083) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in subsets of human PBMCs.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 647) preadsorbed (ab150083) at 1/1000 2 ug/ml dilution.

Confocal image showing membranous staining in subsets of human PBMCs.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)

This data was developed using ab308298, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with ab308298 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor ^® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD3 conjugated to 647. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)

This data was developed using ab308298, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with ab308298 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor ^® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD11b conjugated to BV421. Gated on viable cells.

• WB

Supplier Data

Western blot - Anti-CLEC4A antibody [EPR26969-7] - BSA and Azide free (AB308299)

This data was developed using 308298, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with human CLEC4C. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-CLEC4A antibody [EPR26969-7] (<a href='/en-us/products/primary-antibodies/clec4a-antibody-epr26969-7-ab308298'>ab308298</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human CLEC4A expression vector containi a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Human CLEC4C expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

Exposure time: 180s