AB239077

Anti-CLEC4C antibody [EPR21981]

20ul selling size
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CLEC4C antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD303, BDCA2, CLECSF11, CLECSF7, DLEC, HECL, UNQ9361/PRO34150, CLEC4C, C-type lectin domain family 4 member C, Blood dendritic cell antigen 2, C-type lectin superfamily member 7, Dendritic lectin, BDCA-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (AB239077)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (AB239077)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLEC4C with ab239077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Negative control : Negative staining in human colon (PMID : 11748283).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (AB239077)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC4C with ab239077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with hematoxylin. Membranous staining in plasmacytoid dendritic cells (PDCs) of human tonsil (PMID : 23847189, PMID : 11748283) is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Western blot - Anti-CLEC4C antibody [EPR21981] (AB239077)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular mass at 37-kDa is the glycosylated form, consistent with what has been described in the literature (PMID : 16998465). The 25-kDa band (lane 2) was observed upon deglycosylation.

This blot was developed using a higher sensitivity ECL substrate

All lanes:

Western blot - Anti-CLEC4C antibody [EPR21981] (ab239077) at 1/1000 dilution

Lane 1:

Human tonsil lysate at 20 µg

Lane 2:

Human tonsil lysate treated with PNGase F at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,37 kDa

false

Exposure time: 3min

Carrier free

Anti-CLEC4C antibody [EPR21981] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21981

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells (PubMed : 11748283, PubMed : 21880719, PubMed : 25995448). Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man (PubMed : 21880719, PubMed : 25995448). Binds to serum IgG (PubMed : 25995448). Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells (PubMed : 11748283). May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells (PubMed : 11748283, PubMed : 21880719). May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium (PubMed : 11748283).

See full target information CLEC4C

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0267662 PubMed35476724

2022

Human macrophages and monocyte-derived dendritic cells stimulate the proliferation of endothelial cells through midkine production.

Applications

Unspecified application

Species

Unspecified reactive species

Elias A Said,Sumaya Al-Dughaishi,Wadha Al-Hatmi,Iman Al-Reesi,Marwa Al-Riyami,Mohammed S Al-Balushi,Atika Al-Bimani,Juma Z Al-Busaidi,Murtadha Al-Khabori,Salam Al-Kindi,Francesco A Procopio,Afrah Al-Rashdi,Aliyaa Al-Ansari,Hamza Babiker,Crystal Y Koh,Khalid Al-Naamani,Giuseppe Pantaleo,Ali A Al-Jabri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLEC4C antibody [EPR21981]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (AB239077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (AB239077)

Western blot - Anti-CLEC4C antibody [EPR21981] (AB239077)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise