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AB240576

Anti-CLEC4C antibody [EPR21981] - BSA and Azide free

5

(2 Reviews)

|

(0 Publication)

Rabbit Recombinant Monoclonal CLEC4C antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

CD303, BDCA2, CLECSF11, CLECSF7, DLEC, HECL, UNQ9361/PRO34150, CLEC4C, C-type lectin domain family 4 member C, Blood dendritic cell antigen 2, C-type lectin superfamily member 7, Dendritic lectin, BDCA-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] - BSA and Azide free (AB240576)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] - BSA and Azide free (AB240576)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC4C with ab239077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with hematoxylin. Membranous staining in plasmacytoid dendritic cells (PDCs)of human tonsil (PMID : 23847189, PMID : 11748283) is observed.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239077).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] - BSA and Azide free (AB240576)
  • IHC-P

AbReview83290****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] - BSA and Azide free (AB240576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue staining CLEC4C with ab240576 at 1/100 dilution and co-stained with ab255946. Secondary antibody used was Alexa Fluor® 555 Donkey anti-Rabbit IgG (H+L). The tissue was incubated for 18 hours with PBS +2% normal human serum at 4 degrees. Blocking was done with 5% serum for 1 hour at room temperature. Heat mediated antigen retrieval with Tris-EDTA 1mM PH9.

This image is courtesy of an Abreview submitted by Natalie Papazian

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21981

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab240576 is the carrier-free version of ab239077.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells (PubMed : 11748283, PubMed : 21880719, PubMed : 25995448). Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man (PubMed : 21880719, PubMed : 25995448). Binds to serum IgG (PubMed : 25995448). Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells (PubMed : 11748283). May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells (PubMed : 11748283, PubMed : 21880719). May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium (PubMed : 11748283).
See full target information CLEC4C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com