Rabbit Recombinant Monoclonal CLEC4C antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (AB239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--flow-cytometry-img67142.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (AB239078)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115330.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119480.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102182.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12692.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (AB239078)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/60 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells (PubMed:11748283, PubMed:21880719, PubMed:25995448). Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man (PubMed:21880719, PubMed:25995448). Binds to serum IgG (PubMed:25995448). Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells (PubMed:11748283). May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells (PubMed:11748283, PubMed:21880719). May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium (PubMed:11748283).
Alternative names
CD303, BDCA2, CLECSF11, CLECSF7, DLEC, HECL, UNQ9361/PRO34150, CLEC4C, C-type lectin domain family 4 member C, Blood dendritic cell antigen 2, C-type lectin superfamily member 7, Dendritic lectin, BDCA-2
Recommended products
Rabbit Recombinant Monoclonal CLEC4C antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR21985
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (ab239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--flow-cytometry-img67142.jpg "Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (ab239078)")
Flow Cytometry - Anti-CLEC4C antibody [EPR21985] (ab239078)
Flow cytometric analysis of primary human peripheral blood mononuclear cell (PBMC) labeling CLEC4C with ab239078 at 1/60 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat Anti-Rabbit IgG Fc (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Note: CLEC4C and CD303 are alternative names for the same target.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115330.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining in plasmacytoid dendritic cells (PDCs) of human tonsil (PMID:23847189, PMID:11748283). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119480.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining in plasmacytoid dendritic cells (PDCs) of mouse spleen (PMID:23847189, PMID:11748283). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102182.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining in plasmacytoid dendritic cells (PDCs) of rat spleen (PMID:23847189, PMID:11748283). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078), expandable thumbnail](https://content.abcam.com/products/images/clec4c-antibody-epr21985-ab239078--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12692.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21985] (ab239078)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling CLEC4C with ab239078 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative control: Negative staining in human cardiac muscle (PMID:11748283).
Counter stained with hematoxylin.
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