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AB314871

Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free

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Rabbit Recombinant Monoclonal CLEC5A antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, Dot and reacts with Mouse, Recombinant fragment - Mouse samples.

View Alternative Names

Clecsf5, Mdl1, Clec5a, C-type lectin domain family 5 member A, C-type lectin superfamily member 5, Myeloid DAP12-associating lectin 1, MDL-1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)

This data was developed using ab314870, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CLEC5A with ab314870 at 1/50 (10.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membrane and cytoplasmic staining in RAW 264.7 cell line. Negative control : EL4. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Flow Cytometry - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)

This data was developed using ab314870, the same antibody clone in a different buffer formulation. Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CLEC5A with ab314870 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : EL4. Gated on viable cells.

Flow Cytometry - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)

This data was developed using ab314870, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Mouse splenocytes cells labelling CLEC5A with ab314870 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells. Cells are co-stained with CD11b conjugated to Brilliant Violet™ 421.

Flow Cytometry - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)

This data was developed using ab314870, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Mouse splenocytes cells labelling CLEC5A with ab314870 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

Dot Blot - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)
  • Dot

Supplier Data

Dot Blot - Anti-CLEC5A antibody [EPR28104-14] - BSA and Azide free (AB314871)

This data was developed using ab314870, the same antibody clone in a different buffer formulation. Dot blot analysis of CLEC5A using ab314870 at 1 : 1000 (0.528 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : His-tagged mouse CLEC5A fragment Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Unconjugated

    Anti-CLEC5A antibody [EPR28104-14]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CLEC5A antibody [EPR28104-14]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CLEC5A antibody [EPR28104-14]

  • 578 PE

    PE Anti-CLEC5A antibody [EPR28104-14]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28104-14

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

ICC/IF, Dot, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment - Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

ab314871 is the carrier-free version of ab314870.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a positive regulator of osteoclastogenesis (PubMed : 19251634). Cell surface receptor that signals via TYROBP (PubMed : 10449773). Regulates inflammatory responses (PubMed : 20212065).
See full target information Clec5a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com