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Mouse Monoclonal CLEC9A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CLEC9A protein.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (AB104910), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing CLEC9A protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UXN8

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

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Target data

Function

Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

Alternative names

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Mouse Monoclonal CLEC9A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CLEC9A protein.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing CLEC9A protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UXN8
Clone number

8F9

Purification technique

Affinity purification Protein G

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Growth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (ab104910)

    ICC/IF image of ab104910 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104910, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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