AB104910

Anti-CLEC9A antibody [8F9]

Name: Anti-CLEC9A antibody [8F9] (ab104910) | Abcam
Brand: Abcam Limited
SKU: AB104910
Price: 495 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal CLEC9A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CLEC9A protein.

View Alternative Names

CD370, UNQ9341/PRO34046, CLEC9A, C-type lectin domain family 9 member A

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (AB104910)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (AB104910)

ICC/IF image of ab104910 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104910, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8F9

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Cell preparation containing CLEC9A protein. The exact immunogen used to generate this antibody is proprietary information.

Q6UXN8

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

Growth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

See full target information CLEC9A

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell research 30:763-778 PubMed32541867

2020

Reconstruction of cell spatial organization from single-cell RNA sequencing data based on ligand-receptor mediated self-assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xianwen Ren,Guojie Zhong,Qiming Zhang,Lei Zhang,Yujie Sun,Zemin Zhang

JCI insight 3: PubMed30518680

2018

Enriched Cd141+ DCs in the joint are transcriptionally distinct, activated, and contribute to joint pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mary Canavan,Alice M Walsh,Vipul Bhargava,Sarah M Wade,Trudy McGarry,Viviana Marzaioli,Barry Moran,Monika Biniecka,Hannah Convery,Siobhan Wade,Carl Orr,Ronan Mullan,Jean M Fletcher,Sunil Nagpal,Douglas J Veale,Ursula Fearon

The Journal of clinical investigation 118:2098-110 PubMed18497879

2008

Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin.

Applications

Unspecified application

Species

Human

David Sancho,Diego Mourão-Sá,Olivier P Joffre,Oliver Schulz,Neil C Rogers,Daniel J Pennington,James R Carlyle,Caetano Reis e Sousa

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB104909

Anti-CLEC9A antibody [7H11]

Primary Antibodies

AB104903

Anti-CLEC9A antibody [1F6]

Primary Antibodies

AB222794

Anti-CLEC9A antibody

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody (AB222794)

Primary Antibodies

AB223188

Anti-CLEC9A antibody [EPR22324]

RabMAb|Recombinant

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (AB223188)

Primary Antibodies

AB245121

Anti-CLEC9A antibody [EPR22324] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Complementary Products

Assay Kits

AB309389

Human CLEC9A ELISA Kit

Sandwich ELISA - Human CLEC9A ELISA Kit (AB309389)

Primary Antibodies

AB270797

Anti-CD1c antibody [EPR23189-196] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

Primary Antibodies

AB224202

Anti-CD103 antibody [EPR22590-27]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (AB224202)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com