Rabbit Recombinant Monoclonal CLEC9A antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 6 publications.
Images
![Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (AB223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--flow-cytometry-img66532.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (AB223188)")
![Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (AB223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--flow-cytometry-img66540.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (AB223188)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (AB223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88533.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (AB223188)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (AB223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18551.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (AB223188)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (AB223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (AB223188)")
Publications
Filter by
- Cell reports 40:1112012022Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAthanasios Papadas,Gauri Deb,Alexander Cicala,Adam Officer,Chelsea Hope,Adam Pagenkopf,Evan Flietner,Zachary T Morrow,Philip Emmerich,Joshua Wiesner,Garrett Arauz,Varun Bansal,Karla Esbona,Christian M Capitini,Kristina A Matkowskyj,Dustin A Deming,Katerina Politi,Scott I Abrams,Olivier Harismendy,Fotis AsimakopoulosPubMed 35977482
- Journal of immunology (Baltimore, Md. : 1950) 209:606-6202022An In Vivo Model of Human Macrophages in Metastatic Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentin Voillet,Trisha R Berger,Kelly M McKenna,Kelly G Paulson,Wei Hong Tan,Kimberly S Smythe,Daniel S Hunter,William J Valente,Stephanie Weaver,Jean S Campbell,Teresa S Kim,David R Byrd,Jason H Bielas,Robert H Pierce,Aude G Chapuis,Raphaël Gottardo,Anthony RongvauxPubMed 35817516
- Cancer cell 40:545-557.e132022Ovarian cancer immunogenicity is governed by a narrow subset of progenitor tissue-resident memory T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen M Anadon,Xiaoqing Yu,Kay Hänggi,Subir Biswas,Ricardo A Chaurio,Alexandra Martin,Kyle K Payne,Gunjan Mandal,Patrick Innamarato,Carly M Harro,Jessica A Mine,Kimberly B Sprenger,Carla Cortina,John J Powers,Tara Lee Costich,Bradford A Perez,Chandler D Gatenbee,Sandhya Prabhakaran,Douglas Marchion,Mirjam H M Heemskerk,Tyler J Curiel,Alexander R Anderson,Robert M Wenham,Paulo C Rodriguez,Jose R Conejo-GarciaPubMed 35427494
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e21030292022Smad4 Deficiency Promotes Pancreatic Cancer Immunogenicity by Activating the Cancer-Autonomous DNA-Sensing Signaling Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Xiong,Wenzhuo He,Tiantian Wang,Shuai He,Feifei Xu,Zining Wang,Xiaojuan Wang,Hui Guo,Jianhua Ling,Huanling Zhang,Yongxiang Liu,Kaili Xing,Mengyun Li,Hongxia Zhang,Jiahui Li,Ningning Niu,Jing Xue,Qiuyao Zhan,Ze-Xian Liu,Jin-Xin Bei,Peng Huang,Jinyun Liu,Liangping Xia,Xiaojun XiaPubMed 35064757
- Frontiers in immunology 12:6833962021Characterization of Human Lymphoid Tissue Immune Cells by Multispectral Confocal Imaging and Quantitative Image Analysis; Implications for HIV Reservoir Characterization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEirini Moysi,Perla M Del Rio Estrada,Fernanda Torres-Ruiz,Gustavo Reyes-Terán,Richard A Koup,Constantinos PetrovasPubMed 34177929
- Cell reports. Medicine 2:1001662021A distinct innate immune signature marks progression from mild to severe COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStéphane Chevrier,Yves Zurbuchen,Carlo Cervia,Sarah Adamo,Miro E Raeber,Natalie de Souza,Sujana Sivapatham,Andrea Jacobs,Esther Bachli,Alain Rudiger,Melina Stüssi-Helbling,Lars C Huber,Dominik J Schaer,Jakob Nilsson,Onur Boyman,Bernd BodenmillerPubMed 33521697
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Alternative names
CD370, UNQ9341/PRO34046, CLEC9A, C-type lectin domain family 9 member A
Recommended products
Rabbit Recombinant Monoclonal CLEC9A antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22324
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (ab223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--flow-cytometry-img66532.jpg "Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (ab223188)")
Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (ab223188)
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged CLEC9A overexpression construct, labeling CLEC9A with ab223188 at 1/500 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells.
![Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (ab223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--flow-cytometry-img66540.jpg "Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (ab223188)")
Flow Cytometry - Anti-CLEC9A antibody [EPR22324] (ab223188)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labeling CLEC9A with ab223188 at 1/50 dilution. Cells were stained with Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) or ab223188 (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD3, PE conjugated anti-HLA-DR and BV421 conjugated anti-CD141. Data shown was firstly gated out lymphocytes, then gated on viable CD3(-) HLA-DR (+) APC cells.
CLEC9A is selectively expressed on CD141+DC cells (Q2, ~0.05% in PBMC) and expression pattern was described in literature. (PMID:20479116).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88533.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on dendritic cells (arrows) of human gastric cancer (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18551.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188)
Immunohistochemical analysis of paraffin-embedded human thyoma tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dendritic cells of human thymoma (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr22324-ab223188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] (ab223188)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on dendritic cells of human tonsil (PMID: 18408006; PMID: 20479116) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com