Rabbit Recombinant Monoclonal CLEC9A antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (AB282739), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr24271-2-bsa-and-azide-free-ab282739--flow-cytometry-img172186.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (AB282739)")
![Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (AB282739), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr24271-2-bsa-and-azide-free-ab282739--flow-cytometry-img174907.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (AB282739)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Alternative names
CD370, Dngr-1, Clec9a, C-type lectin domain family 9 member A, Dendritic cell natural killer lectin group receptor 1
Recommended products
Rabbit Recombinant Monoclonal CLEC9A antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24271-2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab282739 is the carrier-free version of Anti-CLEC9A antibody [EPR24271-2] ab270991.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (ab282739), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr24271-2-bsa-and-azide-free-ab282739--flow-cytometry-img172186.jpg "Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (ab282739)")
Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (ab282739)
This data was developed using Anti-CLEC9A antibody [EPR24271-2] ab270991, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of mouse splenocytes cells labelling CLEC9A with Anti-CLEC9A antibody [EPR24271-2] ab270991 at 1/500 dilution (0.1 μg)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or Anti-CLEC9A antibody [EPR24271-2] ab270991, then stained with anti-CD11c conjugated to PE-Cy5.5.
Gated on viable cells.
![Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (ab282739), expandable thumbnail](https://content.abcam.com/products/images/clec9a-antibody-epr24271-2-bsa-and-azide-free-ab282739--flow-cytometry-img174907.jpg "Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (ab282739)")
Flow Cytometry - Anti-CLEC9A antibody [EPR24271-2] - BSA and Azide free (ab282739)
This data was developed using Anti-CLEC9A antibody [EPR24271-2] ab270991, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of mouse splenocytes cells labelling CLEC9A with Anti-CLEC9A antibody [EPR24271-2] ab270991 at 1/500 dilution (0.1 μg)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or Anti-CLEC9A antibody [EPR24271-2] ab270991, then stained with anti-CD8 conjugated to Alexa Fluor® 647 and anti-CD11c conjugated to PE-Cy5.5.
High expression on CD8 positive DC cells.
Gated on CD11c+ viable cells.
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