Rabbit Recombinant Monoclonal CLIC1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Images
![Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--immunoprecipitation-img137331.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514)")
![Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--immunocytochemistry-immunofluorescence-img40540.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514)")
![Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--immunocytochemistry-immunofluorescence-img118474.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514)")
![Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--flow-cytometry-intracellular-img25815.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514)")
![Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--flow-cytometry-intracellular-img25803.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (AB256514)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Not recommended | Tested | Tested |
| Mouse | Tested | Expected | Not recommended | Tested | Tested |
| Rat | Predicted | Expected | Not recommended | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response (PubMed:25581026, PubMed:37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle.
Alternative names
G6, NCC27, CLIC1, Chloride intracellular channel protein 1, Chloride channel ABP, Glutaredoxin-like oxidoreductase CLIC1, Glutathione-dependent dehydroascorbate reductase CLIC1, Nuclear chloride ion channel 27, Regulatory nuclear chloride ion channel protein, hRNCC
Recommended products
Rabbit Recombinant Monoclonal CLIC1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22907-50
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256514 is the carrier-free version of Anti-CLIC1 antibody [EPR22907-50] ab229917.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--immunoprecipitation-img137331.jpg "Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)")
Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)
CLIC1 was immunoprecipitated from 0.35mg Mouse placenta lysate with Anti-CLIC1 antibody [EPR22907-50] ab229917 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CLIC1 antibody [EPR22907-50] ab229917 at 1/1000 dilution (0.5 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Mouse placenta lysate 10μg
Lane 2: Anti-CLIC1 antibody [EPR22907-50] ab229917 IP in Mouse placenta lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CLIC1 antibody [EPR22907-50] ab229917 in Mouse placenta lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLIC1 antibody [EPR22907-50] ab229917).
All lanes: Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] (Anti-CLIC1 antibody [EPR22907-50] ab229917)
Predicted band size: 26 kDa
Observed band size: 27 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--immunocytochemistry-immunofluorescence-img40540.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)")
Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cells labelling CLIC1 with at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in K-562 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLIC1 antibody [EPR22907-50] ab229917).
![Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--immunocytochemistry-immunofluorescence-img118474.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)")
Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labelling CLIC1 with at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in C2C12 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLIC1 antibody [EPR22907-50] ab229917).
![Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--flow-cytometry-intracellular-img25815.jpg "Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)")
Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling CLIC1 with Anti-CLIC1 antibody [EPR22907-50] ab229917 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLIC1 antibody [EPR22907-50] ab229917).
![Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514), expandable thumbnail](https://content.abcam.com/products/images/clic1-antibody-epr22907-50-bsa-and-azide-free-ab256514--flow-cytometry-intracellular-img25803.jpg "Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)")
Flow Cytometry (Intracellular) - Anti-CLIC1 antibody [EPR22907-50] - BSA and Azide free (ab256514)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling CLIC1 with Anti-CLIC1 antibody [EPR22907-50] ab229917 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLIC1 antibody [EPR22907-50] ab229917).
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