AB175230

Anti-CLIC2 antibody [EPR6494]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CLIC2 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Chloride intracellular channel protein 2, Glutaredoxin-like oxidoreductase CLIC2, Glutaredoxin-like peroxidase CLIC2, XAP121, CLIC2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (AB175230)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (AB175230)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (AB175230)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (AB175230)

Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).

Supplier Data

Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (AB175230)

Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (ab175230)

Predicted band size: 28 kDa

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Supplier Data

Western blot - Anti-CLIC2 antibody [EPR6494] (AB175230)

All lanes:

Western blot - Anti-CLIC2 antibody [EPR6494] (ab175230) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Human testis tissue lysate at 10 µg

Predicted band size: 28 kDa

false

Carrier free

Anti-CLIC2 antibody [EPR6494] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6494

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Displays weak glutathione peroxidase activity (Probable) (PubMed : 25581026). Can insert into membranes and form chloride ion channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.

See full target information CLIC2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:89 PubMed40696397

2025

CLIC2 regulates immunosuppression and macrophage differentiation in genomically stable gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Longo,Pellegrino Mazzone,Giovanni Calice,Pietro Zoppoli,Giuseppina Di Paola,Giuseppe Cesta,Margherita Luongo,Claudia Sabato,Sabino Russi,Simona Laurino,Tiziana Notarangelo,Giuseppe Patitucci,Chiara Balzamo,Valeria Lucci,Elena Amendola,Giuseppina Amodio,Paolo Remondelli,Valentina Pagliara,Maria Rita Milone,Roberta Guadagno,Cristofaro De Stefano,Ferdinando De Vita,Geppino Falco,Francesco Albano

Cell reports 41:111766 PubMed36476872

2022

Hippo-released WWC1 facilitates AMPA receptor regulatory complexes for hippocampal learning.

Applications

Unspecified application

Species

Unspecified reactive species

Jens Stepan,Daniel E Heinz,Frederik Dethloff,Thomas Bajaj,Andreas Zellner,Kathrin Hafner,Svenja Wiechmann,Sarah Mackert,Yara Mecdad,Michael Rabenstein,Tim Ebert,Silvia Martinelli,Alexander S Häusl,Maximilian L Pöhlmann,Anke Hermann,Xiao Ma,Hermann Pavenstädt,Mathias V Schmidt,Alexandra Philipsen,Chris W Turck,Jan M Deussing,Bernhard Kuster,Michael C Wehr,Valentin Stein,Joachim Kremerskothen,Carsten T Wotjak,Nils C Gassen

BioMed research international 2022:6496799 PubMed36072472

2022

SLC26A4 Mutation Promotes Cell Apoptosis by Inducing Pendrin Transfer, Reducing Cl Transport, and Inhibiting PI3K/Akt/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Dai,Jun Li,XiJiang Hu,Jian Ye,WenQian Cai

Aging 12:17305-17327 PubMed32915772

2020

Chloride intracellular channel protein 2: prognostic marker and correlation with PD-1/PD-L1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Xu,Zhi Wang,Menglu Dong,Di Wu,Shujie Liao,Xingrui Li

Journal of Cancer 11:3783-3793 PubMed32328183

2020

Analysis of Differentially Expressed Genes in a Chinese Cohort of Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Yuan Zhao,Huili Chen,Jinru Jia,Xiaomin Cheng,Fengjie Wang,Qiang Ji,Rick F Thorne,Song Chen,Xiaoying Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLIC2 antibody [EPR6494]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (AB175230)

Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (AB175230)

Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (AB175230)

Western blot - Anti-CLIC2 antibody [EPR6494] (AB175230)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise