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AB249880

Anti-CLIC2 antibody [EPR6494] - BSA and Azide free

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Rabbit Recombinant Monoclonal CLIC2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

Chloride intracellular channel protein 2, Glutaredoxin-like oxidoreductase CLIC2, Glutaredoxin-like peroxidase CLIC2, XAP121, CLIC2

4 Images
Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)
  • WB

Supplier Data

Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)

This data was developed using ab175230, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CLIC2 antibody [EPR6494] (<a href='/en-us/products/primary-antibodies/clic2-antibody-epr6494-ab175230'>ab175230</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Human testis tissue lysate at 10 µg

Predicted band size: 28 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)

This data was developed using ab175230, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)

This data was developed using ab175230, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)
  • IP

Supplier Data

Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)

This data was developed using ab175230, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (<a href='/en-us/products/primary-antibodies/clic2-antibody-epr6494-ab175230'>ab175230</a>)

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6494

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Displays weak glutathione peroxidase activity (Probable) (PubMed : 25581026). Can insert into membranes and form chloride ion channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
See full target information CLIC2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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