Rabbit Recombinant Monoclonal CLIC2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--immunoprecipitation-img109594.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)")
![Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--western-blot-img119090.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)")
![Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--immunocytochemistry-immunofluorescence-img12555.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129010.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (AB249880)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Displays weak glutathione peroxidase activity (Probable) (PubMed:25581026). Can insert into membranes and form chloride ion channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.
Alternative names
Chloride intracellular channel protein 2, Glutaredoxin-like oxidoreductase CLIC2, Glutaredoxin-like peroxidase CLIC2, XAP121, CLIC2
Recommended products
Rabbit Recombinant Monoclonal CLIC2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6494
- Purification technique
- Affinity purification Protein A
- Specificity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249880 is the carrier-free version of Anti-CLIC2 antibody [EPR6494] ab175230.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--immunoprecipitation-img109594.jpg "Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)")
Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)
This data was developed using Anti-CLIC2 antibody [EPR6494] ab175230, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using Anti-CLIC2 antibody [EPR6494] ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (Anti-CLIC2 antibody [EPR6494] ab175230)
Predicted band size: 28 kDa
![Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--western-blot-img119090.jpg "Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)")
Western blot - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)
This data was developed using Anti-CLIC2 antibody [EPR6494] ab175230, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CLIC2 antibody [EPR6494] (Anti-CLIC2 antibody [EPR6494] ab175230) at 1/1000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: Human heart tissue lysate at 10 µg
Lane 3: Human testis tissue lysate at 10 µg
Predicted band size: 28 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--immunocytochemistry-immunofluorescence-img12555.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)")
Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)
This data was developed using Anti-CLIC2 antibody [EPR6494] ab175230, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling CLIC2 with Anti-CLIC2 antibody [EPR6494] ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880), expandable thumbnail](https://content.abcam.com/products/images/clic2-antibody-epr6494-bsa-and-azide-free-ab249880--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129010.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] - BSA and Azide free (ab249880)
This data was developed using Anti-CLIC2 antibody [EPR6494] ab175230, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with Anti-CLIC2 antibody [EPR6494] ab175230 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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