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AB183043

Anti-CLIC4 antibody [EPR14253]

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(4 Publications)

Rabbit Recombinant Monoclonal CLIC4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Chloride intracellular channel protein 4, Glutaredoxin-like oxidoreductase CLIC4, Intracellular chloride ion channel protein p64H1, CLIC4

6 Images
Flow Cytometry (Intracellular) - Anti-CLIC4 antibody [EPR14253] (AB183043)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CLIC4 antibody [EPR14253] (AB183043)

Intracellular flow cytometric analysis ofRaji cells (paraformaldehyde-fixed, 2%)labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (AB183043)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (AB183043)

Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (AB183043)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLIC4 antibody [EPR14253] (AB183043)

Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (AB183043)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC4 antibody [EPR14253] (AB183043)

Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (AB183043)
  • IP

Supplier Data

Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (AB183043)

Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-CLIC4 antibody [EPR14253] (ab183043)

Predicted band size: 28 kDa

true

Western blot - Anti-CLIC4 antibody [EPR14253] (AB183043)
  • WB

Supplier Data

Western blot - Anti-CLIC4 antibody [EPR14253] (AB183043)

All lanes:

Western blot - Anti-CLIC4 antibody [EPR14253] (ab183043) at 1/10000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

293 lysate at 20 µg

Lane 3:

Raji lysate at 20 µg

Lane 4:

HepG2 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

true

  • Carrier free

    Anti-CLIC4 antibody [EPR14253] - BSA and Azide free

  • 578 PE

    PE Anti-CLIC4 antibody [EPR14253]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14253

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor (PubMed : 25581026, PubMed : 37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions (By similarity) (PubMed : 16176272). Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Promotes cell-surface expression of HRH3.
See full target information CLIC4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:13003-13018 PubMed39413208

2024

SNF2L suppresses nascent DNA gap formation to promote DNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Nelligan,Huzefa Dungrawala

Molecular and cellular biochemistry 476:1783-1795 PubMed33439448

2021

Astragaloside IV alleviates atherosclerosis through targeting circ_0000231/miR-135a-5p/CLIC4 axis in AS cell model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Shao,Zhaozheng Liu,Shanshan Liu,Na Lin,Yue Deng

Clinical proteomics 17:32 PubMed32944011

2020

Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zili Feng,Zhibin Zhu,Wang Chen,Yu Bai,Daihua Hu,Jia Cheng

Biochemical and biophysical research communication 531:490-496 PubMed32807494

2020

Chloride intracellular channel 4 is dysregulated in endometrium of women with infertility and alters receptivity.

Applications

Unspecified application

Species

Unspecified reactive species

Cheuk Kwan Ling,Leilani L Santos,Wei Zhou,Evdokia Dimitriadis
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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