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AB251892

Anti-CLINT1 antibody

5

(4 Reviews)

|

(3 Publications)

Rabbit Polyclonal CLINT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CLINT1 aa 250 to C-terminus.

View Alternative Names

ENTH, EPN4, EPNR, KIAA0171, CLINT1, Clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, Enthoprotin, Epsin-4, Epsin-related protein, Clint, EpsinR

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CLINT1 antibody (AB251892)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CLINT1 antibody (AB251892)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CLINT1 (green) using ab251892 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLINT1 antibody (AB251892)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLINT1 antibody (AB251892)

Paraffin-embedded human colon tissue stained for CLINT1 using ab251892 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-CLINT1 antibody (AB251892)
  • WB

Unknown

Western blot - Anti-CLINT1 antibody (AB251892)

All lanes:

Western blot - Anti-CLINT1 antibody (ab251892) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) whole cell lysate

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CLINT1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14677

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Primary Antibodies

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Primary Antibodies

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Assay Kits

AB245880

H&E Staining Kit (Hematoxylin and Eosin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

  • 5
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
See full target information CLINT1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2021:2408384 PubMed34691176

2021

CircPTK2 (hsa_circ_0003221) Contributes to Laryngeal Squamous Cell Carcinoma by the miR-1278/YAP1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhendong Yang,Jianping Jin,Tao Chang

BMC musculoskeletal disorders 22:62 PubMed33430863

2021

Association of cardiotrophin-like cytokine factor 1 levels in peripheral blood mononuclear cells with bone mineral density and osteoporosis in postmenopausal women.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Chen,Jianyang Li,Yunjin Ye,Jingwen Huang,Lihua Xie,Juan Chen,Shengqiang Li,Sainan Chen,Jirong Ge

Cell transplantation 29:963689720948016 PubMed32787581

2020

Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Lijuan Zhang,Dan Han,Kebinur Tursun,Xiaobo Lu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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