AB134907

Anti-CLIP170 antibody [EPR5093]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CLIP170 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications.

View Alternative Names

CYLN1, RSN, CLIP1, CAP-Gly domain-containing linker protein 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein, Restin, CLIP-170

2 Images

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Western blot - Anti-CLIP170 antibody [EPR5093] (AB134907)

All lanes:

Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

A549 cell lysate at 10 µg

Lane 6:

HUVEC cell lysate at 10 µg

Lane 7:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

Predicted band size: 162 kDa

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Lab

Western blot - Anti-CLIP170 antibody [EPR5093] (AB134907)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody doesn't cross-react with human CLIP3, CLIP4 and CLIP2.

All lanes:

Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907) at 1/1000 dilution

Lane 1:

GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng

Lane 2:

GST-tagged human CLIP4 recombinant full-length protein at 10 ng

Lane 3:

GST-tagged human CLIP2 recombinant full-length protein at 10 ng

Lane 4:

DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 157 kDa

false

Exposure time: 3s

Carrier free

Anti-CLIP170 antibody [EPR5093] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5093

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody doesn't cross-react with human CLIP3, CLIP4 and CLIP2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.

See full target information CLIP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of reproduction and development 71:161-167 PubMed40301068

2025

Mechanism of action of IHH in ameliorating thin endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Luo,Man Luo,Donghong Ning,Xi Chen,Qiuman Zheng,Qin Cao

Nature communications 13:7529 PubMed36477027

2022

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Applications

Unspecified application

Species

Unspecified reactive species

S Mouron,M J Bueno,A Lluch,L Manso,I Calvo,J Cortes,J A Garcia-Saenz,M Gil-Gil,N Martinez-Janez,J V Apala,E Caleiras,Pilar Ximénez-Embún,J Muñoz,L Gonzalez-Cortijo,R Murillo,R Sánchez-Bayona,J M Cejalvo,G Gómez-López,C Fustero-Torre,S Sabroso-Lasa,N Malats,M Martinez,A Moreno,D Megias,M Malumbres,R Colomer,M Quintela-Fandino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLIP170 antibody [EPR5093]

Western blot - Anti-CLIP170 antibody [EPR5093] (AB134907)

Western blot - Anti-CLIP170 antibody [EPR5093] (AB134907)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (2)

Product promise