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Rabbit Recombinant Monoclonal CLIP170 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

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Images

Western blot - Anti-CLIP170 antibody [EPR5093] (AB134907), expandable thumbnail
  • Western blot - Anti-CLIP170 antibody [EPR5093] (AB134907), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.

Alternative names

CYLN1, RSN, CLIP1, CAP-Gly domain-containing linker protein 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein, Restin, CLIP-170

Recommended products

Rabbit Recombinant Monoclonal CLIP170 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5093
Purification technique
Affinity purification Protein A
Specificity

This antibody doesn't cross-react with human CLIP3, CLIP4 and CLIP2

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907), expandable thumbnail

    Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907)

    All lanes: Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: Human fetal kidney tissue lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Lane 5: A549 cell lysate at 10 µg

    Lane 6: HUVEC cell lysate at 10 µg

    Lane 7: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Predicted band size: 162 kDa

  • Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907), expandable thumbnail

    Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907)

    CLIP170 Western blot staining using rabbit Anti-CLIP170 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody doesn't cross-react with human CLIP3, CLIP4 and CLIP2.

    All lanes: Western blot - Anti-CLIP170 antibody [EPR5093] (ab134907) at 1/1000 dilution

    Lane 1: GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng

    Lane 2: GST-tagged human CLIP4 recombinant full-length protein at 10 ng

    Lane 3: GST-tagged human CLIP2 recombinant full-length protein at 10 ng

    Lane 4: DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 157 kDa

    Exposure time: 3s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com