Anti-CLIP170 antibody [EPR5093] - BSA and Azide free

• WB

Unknown

Western blot - Anti-CLIP170 antibody [EPR5093] - BSA and Azide free (AB248720)

This data was developed using ab134907, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CLIP170 antibody [EPR5093] (<a href='/en-us/products/primary-antibodies/clip170-antibody-epr5093-ab134907'>ab134907</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

A549 cell lysate at 10 µg

Lane 6:

HUVEC cell lysate at 10 µg

Lane 7:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

Predicted band size: 162 kDa

false

• WB

Lab

Western blot - Anti-CLIP170 antibody [EPR5093] - BSA and Azide free (AB248720)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134907).

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody doesn't cross-react with human CLIP3, CLIP4 and CLIP2.

All lanes:

Western blot - Anti-CLIP170 antibody [EPR5093] (<a href='/en-us/products/primary-antibodies/clip170-antibody-epr5093-ab134907'>ab134907</a>) at 1/1000 dilution

Lane 1:

GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng

Lane 2:

GST-tagged human CLIP4 recombinant full-length protein at 10 ng

Lane 3:

GST-tagged human CLIP2 recombinant full-length protein at 10 ng

Lane 4:

DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 157 kDa

false

Exposure time: 3s