AB312846

Anti-CLIPR-59 antibody [EPR28002-60]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CLIPR-59 antibody. Suitable for WB, IP and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

View Alternative Names

CLIPR59, CLIP3, CAP-Gly domain-containing linker protein 3, Cytoplasmic linker protein 170-related 59 kDa protein, CLIP-170-related 59 kDa protein, CLIPR-59

6 Images

Supplier Data

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

CLIPR-59 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab312846 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab312846 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat brain tissue lysate 10 ug Lane 2 : Rat brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab312846 in rat brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/30 dilution

All lanes:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Supplier Data

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

CLIPR-59 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab312846 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab312846 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate Lane 2 : Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab312846 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/30 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Supplier Data

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : heart (PMID : 19139280), liver (PMID : 19139280). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 92s

Supplier Data

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (PMID : 19139280). In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

Mouse spinal cord tissue lysate at 50 µg

Lane 2:

Mouse brain tissue lysate at 50 µg

Lane 3:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 60 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

Rat spinal cord tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 60 kDa

false

Exposure time: 92s

Lab

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody doesn't cross-react with human CLIP4, CLIP2 and CLIP1.

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng

Lane 2:

GST-tagged human CLIP4 recombinant full-length protein at 10 ng

Lane 3:

GST-tagged human CLIP2 recombinant full-length protein at 10 ng

Lane 4:

DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 86 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28002-60

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody doesn't cross-react with human CLIP4, CLIP2 and CLIP1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(0.485ug/ml)", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(0.485ug/ml)", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(0.485ug/ml)", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(0.485ug/ml)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. May modulate the cellular compartmentalization of AKT kinase family and promote its cell membrane localization, thereby playing a role in glucose transport in adipocytes.

See full target information CLIP3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLIPR-59 antibody [EPR28002-60]

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise