AB74044

Anti-CLK1 antibody

Name: Anti-CLK1 antibody (ab74044) | Abcam
Brand: Abcam Limited
SKU: AB74044
Rating: 3 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal CLK1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CLK1.

View Alternative Names

CLK, CLK1, Dual specificity protein kinase CLK1, CDC-like kinase 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CLK1 antibody (AB74044)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CLK1 antibody (AB74044)

Immunofluorescence analysis of CLK1 in HUVEC cells, using ab74044 at a 1/500 dilution.
Left image : untreated.
Right image : treated with immunizing peptide.

Unknown

Western blot - Anti-CLK1 antibody (AB74044)

All lanes:

Western blot - Anti-CLK1 antibody (ab74044) at 1/500 dilution

Lane 1:

Extracts from 293 cells at 5 µg

Lane 2:

Extracts from 293 cells at 5 µg with immunizing peptide

Predicted band size: 57 kDa

Observed band size: 30 kDa,34 kDa,36 kDa,57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CLK1. The exact immunogen used to generate this antibody is proprietary information.

P49759

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates : SRSF1, SRSF3 and PTPN1 (PubMed : 10480872, PubMed : 19168442). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (PubMed : 19168442).

See full target information CLK1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:99 PubMed36933158

2023

lncRNA miR4458HG modulates hepatocellular carcinoma progression by activating m6A-dependent glycolysis and promoting the polarization of tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ye,Menghan Wang,Guoyu Wang,Zhongchao Mai,Borong Zhou,Yang Han,Juhua Zhuang,Wei Xia

Genes 13: PubMed36140764

2022

Short-Term Hypoxia in Cells Induces Expression of Genes Which Are Enhanced in Stressed Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Inga Peciuliene,Egle Jakubauskiene,Laurynas Vilys,Ruta Zinkeviciute,Kotryna Kvedaraviciute,Arvydas Kanopka

The Journal of experimental medicine 217: PubMed32453420

2020

Targeting CLK3 inhibits the progression of cholangiocarcinoma by reprogramming nucleotide metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxin Zhou,Meihua Lin,Xing Feng,Fei Ma,Yuekun Zhu,Xing Liu,Chao Qu,Hong Sui,Bei Sun,Anlong Zhu,Heng Zhang,He Huang,Zhi Gao,Yongxiang Zhao,Jiangyun Sun,Yuxian Bai,Junfei Jin,Xuehui Hong,Chang Zou,Zhiyong Zhang

Communications biology 3:268 PubMed32461560

2020

SRPK1 acetylation modulates alternative splicing to regulate cisplatin resistance in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Zhihong Zhou,Charannya Sozheesvari Subhramanyam,Qiong Cao,Zealyn Shi Lin Heng,Wen Liu,Xiangdong Fu,Qidong Hu

Molecular pharmacology 85:441-50 PubMed24366666

2013

cdc-like/dual-specificity tyrosine phosphorylation-regulated kinases inhibitor leucettine L41 induces mTOR-dependent autophagy: implication for Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Fant,Emilie Durieu,Gaëtan Chicanne,Bernard Payrastre,Diego Sbrissa,Assia Shisheva,Emmanuelle Limanton,François Carreaux,Jean-Pierre Bazureau,Laurent Meijer

The Journal of cell biology 195:27-40 PubMed21949414

2011

Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor.

Applications

Species

Unspecified reactive species

Kensuke Ninomiya,Naoyuki Kataoka,Masatoshi Hagiwara

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLK1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CLK1 antibody (AB74044)

Western blot - Anti-CLK1 antibody (AB74044)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Biochemicals

Product promise