Rabbit Recombinant Monoclonal CLP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-CLP1 antibody [EPR7180(2)] (AB133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--western-blot-img105318.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLP1 antibody [EPR7180(2)] (AB133669)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (AB133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33577.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (AB133669)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (AB133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119705.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (AB133669)")
![OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (AB133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--oi-rd-scanning-img95495.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (AB133669)")
Publications
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- Neuron 110:1340-1357.e72022Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeneva R LaForce,Jordan S Farr,Jingyi Liu,Cydni Akesson,Evren Gumus,Otis Pinkard,Helen C Miranda,Katherine Johnson,Thomas J Sweet,Ping Ji,Ai Lin,Jeff Coller,Polyxeni Philippidou,Eric J Wagner,Ashleigh E SchafferPubMed 35139363
- Molecular cell 77:1206-1221.e72020A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeung Wook Yang et. al.PubMed 31980388
- Cell 157:651-632014CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration.Applications:IF, WBReactive species:Unspecified reactive speciesAshleigh E Schaffer,Veerle R C Eggens,Ahmet Okay Caglayan,Miriam S Reuter,Eric Scott,Nicole G Coufal,Jennifer L Silhavy,Yuanchao Xue,Hulya Kayserili,Katsuhito Yasuno,Rasim Ozgur Rosti,Mostafa Abdellateef,Caner Caglar,Paul R Kasher,J Leonie Cazemier,Marian A Weterman,Vincent Cantagrel,Na Cai,Christiane Zweier,Umut Altunoglu,N Bilge Satkin,Fesih Aktar,Beyhan Tuysuz,Cengiz Yalcinkaya,Huseyin Caksen,Kaya Bilguvar,Xiang-Dong Fu,Christopher R Trotta,Stacey Gabriel,André Reis,Murat Gunel,Frank Baas,Joseph G GleesonPubMed 24766810
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Alternative names
HEAB, CLP1, Polyribonucleotide 5'-hydroxyl-kinase Clp1, Polyadenylation factor Clp1, Polynucleotide kinase Clp1, Pre-mRNA cleavage complex II protein Clp1
Recommended products
Rabbit Recombinant Monoclonal CLP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7180(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 8.5 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--western-blot-img105318.jpg "Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)")
Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
All lanes: Western blot - Anti-CLP1 antibody [EPR7180(2)] (ab133669) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 48 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33577.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CLP1 with ab133669 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119705.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CLP1 with ab133669 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669), expandable thumbnail](https://content.abcam.com/products/images/clp1-antibody-epr71802-ab133669--oi-rd-scanning-img95495.jpg "OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669)")
OI-RD Scanning - Anti-CLP1 antibody [EPR7180(2)] (ab133669)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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