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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal CLPP antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 40 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | IP | Flow Cyt | WB | |
---|---|---|---|---|
Human | Tested | Tested | Not recommended | Tested |
Mouse | Tested | Expected | Not recommended | Tested |
Rat | Tested | Expected | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates (PubMed:11923310, PubMed:15522782). Cleaves PINK1 in the mitochondrion (PubMed:22354088).
Endopeptidase Clp, CLPP
Rabbit Recombinant Monoclonal CLPP antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 40 publications.
Endopeptidase Clp, CLPP
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR7133
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-CLPP antibody [EPR7133] (AB124822) at 0.05 µg/mL
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 15 µg
Lane 3: Mouse brain lysates at 15 µg
Lane 4: Rat brain lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
ab124822 (purified) at 1:20 dilution (2μg) immunoprecipitating CLPP in K562 whole cell lysate.
Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
Lane 2 (+): ab124822 & K562 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-CLPP antibody [EPR7133] (AB124822)
Predicted band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
All lanes: Western blot - Anti-CLPP antibody [EPR7133] (AB124822) at 1/1000 dilution
Lane 1: Fetal muscle lysate at 10 µg
Lane 2: Saos-2 (Human osteosarcoma cell line) cell lysate at 10 µg
Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg
Lane 5: K562 cell lysate at 10 µg
Lane 6: A549 (Human lung carcinoma cell line) cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 26 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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