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Rabbit Recombinant Monoclonal CLPP antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 40 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Tested
Not recommended
Tested
Mouse
Tested
Expected
Not recommended
Tested
Rat
Tested
Expected
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Target data

Function

Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates (PubMed:11923310, PubMed:15522782). Cleaves PINK1 in the mitochondrion (PubMed:22354088).

Alternative names

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Rabbit Recombinant Monoclonal CLPP antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 40 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7133

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CLPP antibody [EPR7133] (ab124822), expandable thumbnail

    Western blot - Anti-CLPP antibody [EPR7133] (ab124822)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-CLPP antibody [EPR7133] (AB124822) at 0.05 µg/mL

    Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

    Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 3: Mouse brain lysates at 15 µg

    Lane 4: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] (ab124822), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] (ab124822)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-CLPP antibody [EPR7133] (ab124822), expandable thumbnail

    Immunoprecipitation - Anti-CLPP antibody [EPR7133] (ab124822)

    ab124822 (purified) at 1:20 dilution (2μg) immunoprecipitating CLPP in K562 whole cell lysate.
    Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
    Lane 2 (+): ab124822 & K562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CLPP antibody [EPR7133] (AB124822)

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] (ab124822), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] (ab124822)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] (ab124822), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] (ab124822)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Western blot - Anti-CLPP antibody [EPR7133] (ab124822), expandable thumbnail

    Western blot - Anti-CLPP antibody [EPR7133] (ab124822)

    All lanes: Western blot - Anti-CLPP antibody [EPR7133] (AB124822) at 1/1000 dilution

    Lane 1: Fetal muscle lysate at 10 µg

    Lane 2: Saos-2 (Human osteosarcoma cell line) cell lysate at 10 µg

    Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

    Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

    Lane 5: K562 cell lysate at 10 µg

    Lane 6: A549 (Human lung carcinoma cell line) cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa

    Observed band size: 26 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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