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Rabbit Recombinant Monoclonal CLPP antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064), expandable thumbnail
  • Immunoprecipitation - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064), expandable thumbnail
  • Western blot - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064), expandable thumbnail

Publications

  • Haematologica 104:1756-17672019
    Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Nunziata Maio et. al.
    PubMed 30765471

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

    8 products for Alternative Version

      Target data

      Function

      Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates (PubMed:11923310, PubMed:15522782). Cleaves PINK1 in the mitochondrion (PubMed:22354088).

      Alternative names

      Endopeptidase Clp, CLPP

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      Rabbit Recombinant Monoclonal CLPP antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

      Alternative names

      Endopeptidase Clp, CLPP

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR7133

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab236064 is the carrier-free version of Anti-CLPP antibody [EPR7133] ab124822.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified Anti-CLPP antibody [EPR7133] ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLPP antibody [EPR7133] ab124822).

      • Immunoprecipitation - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064), expandable thumbnail

        Immunoprecipitation - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

        Anti-CLPP antibody [EPR7133] ab124822 (purified) at 1:20 dilution (2μg) immunoprecipitating CLPP in K562 whole cell lysate.
        Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
        Lane 2 (+): Anti-CLPP antibody [EPR7133] ab124822 & K562 whole cell lysate
        Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CLPP antibody [EPR7133] ab124822 in K562 whole cell lysate
        For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:5000 dilution.
        Blocking and diluting buffer: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLPP antibody [EPR7133] ab124822).

        All lanes: Immunoprecipitation - Anti-CLPP antibody [EPR7133] (Anti-CLPP antibody [EPR7133] ab124822)

        Predicted band size: 30 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified Anti-CLPP antibody [EPR7133] ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLPP antibody [EPR7133] ab124822).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified Anti-CLPP antibody [EPR7133] ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLPP antibody [EPR7133] ab124822).

      • Western blot - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064), expandable thumbnail

        Western blot - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CLPP antibody [EPR7133] ab124822).

        All lanes: Western blot - Anti-CLPP antibody [EPR7133] (Anti-CLPP antibody [EPR7133] ab124822) at 1/1000 dilution

        Lane 1: Fetal muscle lysate at 10 µg

        Lane 2: Saos-2 (Human osteosarcoma cell line) cell lysate at 10 µg

        Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

        Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

        Lane 5: K562 cell lysate at 10 µg

        Lane 6: A549 (Human lung carcinoma cell line) cell lysate at 10 µg

        Secondary

        All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 30 kDa

        Observed band size: 26 kDa

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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