Anti-CLPP antibody [EPR7133] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1 : 50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

• IP

Unknown

Immunoprecipitation - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064)

ab124822 (purified) at 1 : 20 dilution (2μg) immunoprecipitating CLPP in K562 whole cell lysate.
Lane 1 (input) : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
Lane 2 (+) : ab124822 & K562 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 5000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

All lanes:

Immunoprecipitation - Anti-CLPP antibody [EPR7133] (<a href='/en-us/products/primary-antibodies/clpp-antibody-epr7133-ab124822'>ab124822</a>)

Predicted band size: 30 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1 : 50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1 : 50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

• WB

Unknown

Western blot - Anti-CLPP antibody [EPR7133] - BSA and Azide free (AB236064)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

All lanes:

Western blot - Anti-CLPP antibody [EPR7133] (<a href='/en-us/products/primary-antibodies/clpp-antibody-epr7133-ab124822'>ab124822</a>) at 1/1000 dilution

Lane 1:

Fetal muscle lysate at 10 µg

Lane 2:

Saos-2 (Human osteosarcoma cell line) cell lysate at 10 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 5:

K562 cell lysate at 10 µg

Lane 6:

A549 (Human lung carcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 30 kDa

Observed band size: 26 kDa

false