Rabbit Recombinant Monoclonal CLSTN1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (AB134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--immunocytochemistry-immunofluorescence-img65158.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (AB134130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (AB134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95593.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (AB134130)")
![Western blot - Anti-CLSTN1 antibody [EPR2963] (AB134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--western-blot-img13868.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLSTN1 antibody [EPR2963] (AB134130)")
![Western blot - Anti-CLSTN1 antibody [EPR2963] (AB134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--western-blot-img142758.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CLSTN1 antibody [EPR2963] (AB134130)")
Publications
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- Cell reports 35:1092902021The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Yi Ou,Qi Xiao,Xiang-Chun Ju,Peng-Ming Zeng,Jing Huang,Ai-Li Sheng,Zhen-Ge LuoPubMed 34192541
- Molecular & cellular proteomics : MCP 18:546-5602019Changes in Synaptic Proteins Precede Neurodegeneration Markers in Preclinical Alzheimer's Disease Cerebrospinal Fluid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Lleó et. al.PubMed 30606734
- PloS one 11:e01583472016The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yu,Xuejun Gong,Lei Sun,Qiying Zhou,Baoling Lu,Liying ZhuPubMed 27391479
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Expected | Not recommended | Not recommended | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes - |
Target data
Function
Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (By similarity). Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane (By similarity). Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles (PubMed:21385839). Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (PubMed:12972431). Soluble Alc-alpha. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding (PubMed:15037614). CTF1-alpha. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP (PubMed:15037614).
Alternative names
CS1, KIAA0911, CLSTN1, Calsyntenin-1, Alcadein-alpha, Alzheimer-related cadherin-like protein, Non-classical cadherin XB31alpha, Alc-alpha
Recommended products
Rabbit Recombinant Monoclonal CLSTN1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2963
- Purification technique
- Affinity purification Protein A
- Specificity
The immunogen used for this product shares 91% homology with CLSTN2. Cross-reactivity with this protein has not been confirmed experimentally.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--immunocytochemistry-immunofluorescence-img65158.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)")
Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)
Immfluorescent staining of paraffin embedded Human SH SY 5Y cells labelling CLSTN1 with ab134130 at 1/100 dilution
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95593.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CLSTN1 with ab134130 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--western-blot-img13868.jpg "Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)")
Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
All lanes: Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: U87 MG lysate at 10 µg
Lane 4: SH SY5Y lysate at 10 µg
Lane 5: Neuro 2a lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti mouse at 1/2000 dilution
Predicted band size: 110 kDa
![Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130), expandable thumbnail](https://content.abcam.com/products/images/clstn1-antibody-epr2963-ab134130--western-blot-img142758.jpg "Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)")
Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
Blocking and diluting buffer: 5% NFDM/TBST
Extra band at: 90kDa We are unsure as to the identity of this extra band.
All lanes: Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/200000 dilution
All lanes: Human fetal brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: 3min
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