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AB248693

Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free

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Rabbit Recombinant Monoclonal CLSTN1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples.

View Alternative Names

CS1, KIAA0911, CLSTN1, Calsyntenin-1, Alcadein-alpha, Alzheimer-related cadherin-like protein, Non-classical cadherin XB31alpha, Alc-alpha

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)

This data was developed using ab134130, the same antibody clone in a different buffer formulation.Immfluorescent staining of paraffin embedded Human SH SY 5Y cells labelling CLSTN1 with ab134130 at 1/100 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)

This data was developed using ab134130, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CLSTN1 with ab134130 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)
  • WB

Lab

Western blot - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)

This data was developed using ab134130, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Extra band at : 90kDa. We are unsure as to the identity of this extra band.

All lanes:

Western blot - Anti-CLSTN1 antibody [EPR2963] (<a href='/en-us/products/primary-antibodies/clstn1-antibody-epr2963-ab134130'>ab134130</a>) at 1/200000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 150 kDa

false

Exposure time: 3min

Western blot - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)
  • WB

Unknown

Western blot - Anti-CLSTN1 antibody [EPR2963] - BSA and Azide free (AB248693)

This data was developed using ab134130, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CLSTN1 antibody [EPR2963] (<a href='/en-us/products/primary-antibodies/clstn1-antibody-epr2963-ab134130'>ab134130</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

U87 MG lysate at 10 µg

Lane 4:

SH SY5Y lysate at 10 µg

Lane 5:

Neuro 2a lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti mouse at 1/2000 dilution

Predicted band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2963

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 91% homology with CLSTN2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248693 is the carrier-free version of ab134130.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (By similarity). Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane (By similarity). Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles (PubMed : 21385839). Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation (PubMed : 12972431).. Soluble Alc-alpha. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding (PubMed : 15037614).. CTF1-alpha. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP (PubMed : 15037614).
See full target information CLSTN1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com