Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Rabbit Recombinant Monoclonal Clusterin antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-Fr	IHC-P	ICC/IF
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Expected	Tested	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681).
Species Rat	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681).

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/8000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/8000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse	Dilution info -	Notes -

Target data

See full target information Clu

Function

Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins (By similarity). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed:14741101). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity (By similarity). Following stress, promotes apoptosis (PubMed:12551933). Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. Following ER stress, suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. When secreted, does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (By similarity). When secreted, acts as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (PubMed:11865066).

Alternative names

Apoj, Msgp-2, Clu, Clusterin, Apolipoprotein J, Clustrin, Sulfated glycoprotein 2, Apo-J, SGP-2

Recommended products

Rabbit Recombinant Monoclonal Clusterin antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17539-95
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Clusterin also called apolipoprotein J has an important role in various biological processes. The clusterin alpha chain which is a segment of the clusterin protein is synthesized as part of a precursor that is then cleaved into alpha and beta chains. The mass of this alpha chain is around 34 kDa. Clusterin is expressed in multiple tissues including the liver brain and testes. It often appears in bodily fluids such as plasma and cerebrospinal fluid.

Biological function summary

The clusterin protein operates as a chaperone that prevents non-specific aggregation of proteins which is important in maintaining cellular protein homeostasis. It forms part of a heterodimeric complex composed of alpha and beta chains. This protein also contributes to lipid transport due to its apolipoprotein nature. Its expression levels can increase in response to stress-related conditions suggesting an adaptive function to protect cells.

Pathways

Clusterin is involved in the regulation of cell death and survival pathways. It plays a role in the complement cascade providing protective effects against membrane attack complexes. Clusterin interacts with other proteins such as CRP which assists in modulating these pathways and influences inflammation as part of the immune response.

Associated diseases and disorders

Disruption of clusterin expression or function relates to neurodegenerative disorders including Alzheimer's disease. The clusterin protein associates with amyloid-beta affecting its aggregation and clearance. It also connects to prostate cancer where altered expression of clusterin might influence tumor cell survival and treatment resistance. Understanding these interactions might offer potential therapeutic strategies with clusterin acting as a target or biomarker in diagnostics or treatment development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure times:
Lanes 1,3,4: 24 seconds;
Lane 2: 3 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 8373947).
The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument
All lanes: Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100) at 1/1000 dilution
Lane 1: Mouse serum lysate at 20 µg
Lane 2: Rat serum lysate at 10 µg
Lane 3: Mouse lung lysate at 10 µg
Lane 4: Mouse adrenal gland lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of rat adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of mouse adrenal gland than the cortex (PMID: 16436671) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of frozen rat brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in rat brain (PMID:18620027; 21385939) is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells (PMID: 22552734) is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Clusterin alpha chain was immunoprecipitated from 0.35 mg mouse serum lysate with ab184100 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184100 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Mouse serum lysate 10 μl (Input).
Lane 2: ab184100 IP in mouse serum lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184100 in mouse serum lysate (-).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Predicted band size: 52 kDa
Observed band size: 38 kDa
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in mouse brain (PMID:18620027; 21385939) is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)
Immunohistochemical analysis of 4% paraformaldehude-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells is observed; also observe particles deposited on sperm membrane (PMID: 22552734).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).