Anti-Clusterin alpha chain antibody [EPR17539-95]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of rat adrenal gland than the cortex (PMID : 16436671) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat spleen (PMID : 24865838) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in mouse brain (PMID : 18620027; 21385939) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells (PMID : 22552734) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of frozen rat brain (lateral ventricle) tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in endothelial cells of choroid plexus and astrocytes in rat brain (PMID : 18620027; 21385939) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of 4% paraformaldehude-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling Clusterin alpha chain with ab184100 at 1/250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in Sertoli cells, immature spermatozoa and Leydig cells is observed; also observe particles deposited on sperm membrane (PMID : 22552734).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla of mouse adrenal gland than the cortex (PMID : 16436671) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin alpha chain with ab184100 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse spleen (PMID : 24865838) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

Supplier Data

Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Clusterin alpha chain was immunoprecipitated from 0.35 mg mouse serum lysate with ab184100 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184100 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Mouse serum lysate 10 μl (Input).
Lane 2 : ab184100 IP in mouse serum lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184100 in mouse serum lysate (-).

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100)

Predicted band size: 52 kDa

Observed band size: 38 kDa

false

• WB

Supplier Data

Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (AB184100)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Exposure times :

Lanes 1,3,4 : 24 seconds;

Lane 2 : 3 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 8373947).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument

All lanes:

Western blot - Anti-Clusterin alpha chain antibody [EPR17539-95] (ab184100) at 1/1000 dilution

Lane 1:

Mouse serum lysate at 20 µg

Lane 2:

Rat serum lysate at 10 µg

Lane 3:

Mouse lung lysate at 10 µg

Lane 4:

Mouse adrenal gland lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

true