Anti-Clusterin antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB79280)

HeLa cells stained for Clusterin (green) using ab79280 at 10 μg/ml in ICC/IF. Cells were permeabilized with 0.15% Triton. DAPI was used as a nuclear counterstain.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (AB79280)

Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr using ab79280 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• WB

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Western blot - Anti-Clusterin antibody (AB79280)

All lanes:

Western blot - Anti-Clusterin antibody (ab79280) at 0.3 µg/mL

All lanes:

Human Testis lysate at 35 µg

Predicted band size: 52 kDa

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• WB

Unknown

Western blot - Anti-Clusterin antibody (AB79280)

The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12) : 1407-12.; PMID : 11560857).

All lanes:

Western blot - Anti-Clusterin antibody (ab79280) at 0.3 µg/mL

All lanes:

Rat Brain lysate (in RIPA buffer) at 35 µg

Predicted band size: 52 kDa

Observed band size: 37 kDa

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