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Anti-Clusterin antibody [EPR2911] (ab92548) is a rabbit monoclonal antibody detecting Clusterin in Western Blot, IHC-P. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Trusted since 2010


Images

Western blot - Anti-Clusterin antibody [EPR2911] (AB92548), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548), expandable thumbnail
  • Western blot - Anti-Clusterin antibody [EPR2911] (AB92548), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548), expandable thumbnail
  • Western blot - Anti-Clusterin antibody [EPR2911] (AB92548), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/450
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Isoform 1. Functions as extracellular chaperone that prevents aggregation of non native proteins (PubMed:11123922, PubMed:19535339). Prevents stress-induced aggregation of blood plasma proteins (PubMed:11123922, PubMed:12176985, PubMed:17260971, PubMed:19996109). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed:12047389, PubMed:17407782, PubMed:17412999). Does not require ATP (PubMed:11123922). Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70 (PubMed:11123922). Does not refold proteins by itself (PubMed:11123922). Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation (PubMed:21505792). Protects cells against apoptosis and against cytolysis by complement (PubMed:2780565). Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:20068069). Promotes proteasomal degradation of COMMD1 and IKBKB (PubMed:20068069). Modulates NF-kappa-B transcriptional activity (PubMed:12882985). A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis (PubMed:16113678, PubMed:17689225). Plays a role in the regulation of cell proliferation (PubMed:19137541). An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5 (PubMed:22689054). Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed:24073260). Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (By similarity). Isoform 6. Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Isoform 4. Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed:24073260). Promotes cell death through interaction with BCL2L1 that releases and activates BAX (PubMed:21567405).

Alternative names

APOJ, CLI, KUB1, AAG4, CLU, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2, Apo-J, SGP-2, TRPM-2

Recommended products

Anti-Clusterin antibody [EPR2911] (ab92548) is a rabbit monoclonal antibody detecting Clusterin in Western Blot, IHC-P. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Trusted since 2010

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2911
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Clusterin antibody [EPR2911] (ab92548) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Clusterin?


Anti-Clusterin [EPR2911] (ab92548) specifically detects a band for Clusterin (UniProt: P10909) at a molecular weight of 52kDa.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-Clusterin antibody [EPR2911] (ab92548) has been confirmed by Western blot testing in CLU Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR2911] also available for your convenience:
ab92548, Carrier free - Anti-Clusterin antibody [EPR2911] - Low endotoxin, Azide free ab229445, Carrier free - Anti-Clusterin antibody [EPR2911] - BSA and Azide free ab271867, APC - APC Anti-Clusterin antibody [EPR2911] ab319236, PE - PE Anti-Clusterin antibody [EPR2911] ab319384, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Clusterin antibody [EPR2911] ab319518, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Clusterin antibody [EPR2911] ab319631, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Clusterin antibody [EPR2911] ab319735, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Clusterin antibody [EPR2911] ab319877, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Clusterin antibody [EPR2911] ab321764



Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.

Biological function summary

Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.

Pathways

Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.

Associated diseases and disorders

Scientists associate clusterin with Alzheimer’s disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.

Product promise

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7 product images

  • Western blot - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Clusterin knockout HAP1 whole cell lysate (20 μg)
    Lane 3: U87-MG whole cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab92548 observed at 68 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab92548 was shown to recognize Clusterin in wild-type HAP1 cells as signal was lost at the expected MW in Clusterin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Clusterin knockout samples were subjected to SDS-PAGE. ab92548 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    Lane 1: Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

    Lanes 2 - 3: Western blot - Anti-Clusterin antibody [EPR2911] - Low endotoxin, Azide free (Anti-Clusterin antibody [EPR2911] - Low endotoxin, Azide free ab229445) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: Clusterin knockout HAP1 whole cell lysate at 20 µg

    Lane 3: U87-MG whole cell lysate at 20 µg

    Predicted band size: 52 kDa

    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Clusterin with purified ab92548 at 1/450 dilution (0.23 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

    Clusterin precursor could be cleaved to produce an N-terminal α-chain and a C-terminal β-chain as was described by PMID: 25402950. ab92548 recognizes α-chain.

    All lanes: Western blot - Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

    Lane 1: Human testis lysates at 20 µg

    Lane 2: Human brain lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 52 kDa

    Observed band size: 37 kDa, 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548)

    ab92548 (unpurified) at 1/100 dilution staining Apolipoprotein J in Human testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

    All lanes: Western blot - Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 10 µg

    Lane 2: Fetal brain tissue lysate at 10 µg

    Lane 3: Human Plasma lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • Western blot - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

    Clusterin Western blot staining using rabbit Anti-Clusterin antibody

    Western blot: Rabbit Monoclonal[EPR2911] to Clusterin ab92548 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 60 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in CLU knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG at 20 µg

    Lane 2: Human CLU knockout U-87 MG cell line at 20 µg

    Lane 3: Wild-type HAP1 at 20 µg

    Lane 4: CLU knockout HAP1 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548)

    Clusterin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human Alzheimer's brain tissue using rabbit Anti-Clusterin antibody

    Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain tissue* labelling CLU with ab92548 at 0.1ug/ml followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human Alzheimer's brain.

    The section was incubated with ab92548 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution1) for 20 mins.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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