AB92548

Anti-Clusterin antibody [EPR2911]

Name: Anti-Clusterin antibody [EPR2911] (ab92548) | Abcam
Brand: Abcam Limited
SKU: AB92548
Price: 220 USD
Availability: InStock
Rating: 4 (3 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

(3 Reviews)

(13 Publications)

Anti-Clusterin antibody [EPR2911] (ab92548) is a rabbit monoclonal antibody detecting Clusterin in Western Blot, IHC-P. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2010

View Alternative Names

APOJ, CLI, KUB1, AAG4, CLU, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2, Apo-J, SGP-2, TRPM-2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Clusterin with purified ab92548 at 1/450 dilution (0.23 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

ab92548 (unpurified) at 1/100 dilution staining Apolipoprotein J in Human testis tissue by Immunohistochemistry Formalin-fixed Paraffin-embedded tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain tissue* labelling CLU with ab92548 at 0.1ug/ml followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining in human Alzheimer's brain.

The section was incubated with ab92548 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution1) for 20 mins.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Lab

Western blot - Anti-Clusterin antibody [EPR2911] (AB92548)

Clusterin precursor could be cleaved to produce an N-terminal Î±-chain and a C-terminal Î²-chain as was described by PMID : 25402950. ab92548 recognizes α-chain.

All lanes:

Western blot - Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

Lane 1:

Human testis lysates at 20 µg

Lane 2:

Human brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 37 kDa,68 kDa

false

Unknown

Western blot - Anti-Clusterin antibody [EPR2911] (AB92548)

All lanes:

Western blot - Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

Human Plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

false

Carrier free

Anti-Clusterin antibody [EPR2911] - BSA and Azide free
578 PE

PE Anti-Clusterin antibody [EPR2911]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Clusterin antibody [EPR2911]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Clusterin antibody [EPR2911]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Clusterin antibody [EPR2911]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Clusterin antibody [EPR2911]
660 APC

APC Anti-Clusterin antibody [EPR2911]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Clusterin antibody [EPR2911]
Carrier free

Anti-Clusterin antibody [EPR2911] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2911

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/450", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Clusterin antibody [EPR2911] (ab92548) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Clusterin?
Anti-Clusterin [EPR2911] (ab92548) specifically detects a band for Clusterin (UniProt: P10909) at a molecular weight of 52kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Clusterin antibody [EPR2911] (ab92548) has been confirmed by Western blot testing in CLU Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR2911] also available for your convenience: ab92548, Carrier free - ab229445, Carrier free - ab271867, APC - ab319236, PE - ab319384, Alexa Fluor® 488 - ab319518, Alexa Fluor® 647 - ab319631, Alexa Fluor® 594 - ab319735, Alexa Fluor® 555 - ab319877, Alexa Fluor® 750 - ab321764

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.

Biological function summary

Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.

Pathways

Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.

Scientists associate clusterin with Alzheimer's disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Functions as extracellular chaperone that prevents aggregation of non native proteins (PubMed : 11123922, PubMed : 19535339). Prevents stress-induced aggregation of blood plasma proteins (PubMed : 11123922, PubMed : 12176985, PubMed : 17260971, PubMed : 19996109). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed : 12047389, PubMed : 17407782, PubMed : 17412999). Does not require ATP (PubMed : 11123922). Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70 (PubMed : 11123922). Does not refold proteins by itself (PubMed : 11123922). Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation (PubMed : 21505792). Protects cells against apoptosis and against cytolysis by complement : inhibits assembly of the complement membrane attack complex (MAC) by preventing polymerization of C9 pore component of the MAC complex (PubMed : 2780565, PubMed : 1903064, PubMed : 2601725, PubMed : 2721499, PubMed : 1551440, PubMed : 9200695, PubMed : 34667172). Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 20068069). Promotes proteasomal degradation of COMMD1 and IKBKB (PubMed : 20068069). Modulates NF-kappa-B transcriptional activity (PubMed : 12882985). A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis (PubMed : 16113678, PubMed : 17689225). Plays a role in the regulation of cell proliferation (PubMed : 19137541). An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5 (PubMed : 22689054). Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed : 24073260). Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (By similarity).. Isoform 6. Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity.. Isoform 4. Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed : 24073260). Promotes cell death through interaction with BCL2L1 that releases and activates BAX (PubMed : 21567405).

See full target information CLU

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:188 PubMed40898372

2025

Optineurin deficiency disrupts phosphorylated tau proteostasis and clusterin expression in human neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary M Augur,Garrett M Fogo,Mason R Arbery,Andrew M Stern,Courtney R Benoit,Yi-Chen Hsieh,Tracy L Young-Pearse

Aging cell 23:e14320 PubMed39158018

2024

Single-cell RNA sequencing reveals the CRTAC1 population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Tang,Dachun Zhuo,Yuexin Yu,Weilin Pu,Yanyun Ma,Yuting Zhang,Yan Huang,Qing Zhang,Kunhai Tang,Chen Meng,Di Yang,Lu Bai,Dongyi He,Li Jin,Hejian Zou,Huji Xu,Qi Zhu,Jiucun Wang,Yuanyuan Chen,Jing Liu

Cell reports 42:112994 PubMed37611586

2023

Cell-type-specific regulation of APOE and CLU levels in human neurons by the Alzheimer's disease risk gene SORL1.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo Lee,Aimee J Aylward,Richard V Pearse,Alexandra M Lish,Yi-Chen Hsieh,Zachary M Augur,Courtney R Benoit,Vicky Chou,Allison Knupp,Cheryl Pan,Srilakshmi Goberdhan,Duc M Duong,Nicholas T Seyfried,David A Bennett,Mariko F Taga,Kevin Huynh,Matthias Arnold,Peter J Meikle,Philip L De Jager,Vilas Menon,Jessica E Young,Tracy L Young-Pearse

Cancers 14: PubMed35626071

2022

Cytoplasmic Clusterin Suppresses Lung Cancer Metastasis by Inhibiting the ROCK1-ERK Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaobo Huang,Xu Li,Weiqi Gu,Xiaoyi Li,Jingjing Zhao,Jueheng Wu,Junchao Cai,Xianming Feng,Tianyu Tao

Kidney international 101:137-143 PubMed34619231

2021

Highly multiplexed immunofluorescence of the human kidney using co-detection by indexing.

Applications

mIHC

Species

Human

Elizabeth K Neumann,Nathan Heath Patterson,Emilio S Rivera,Jamie L Allen,Maya Brewer,Mark P deCaestecker,Richard M Caprioli,Agnes B Fogo,Jeffrey M Spraggins

Nature communications 12:4863 PubMed34381050

2021

The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Yuste-Checa,Victoria A Trinkaus,Irene Riera-Tur,Rahmi Imamoglu,Theresa F Schaller,Huping Wang,Irina Dudanova,Mark S Hipp,Andreas Bracher,F Ulrich Hartl

Genome biology 22:156 PubMed34001209

2021

A systematic dissection of the epigenomic heterogeneity of lung adenocarcinoma reveals two different subclasses with distinct prognosis and core regulatory networks.

Applications

Unspecified application

Species

Unspecified reactive species

Chongze Yuan,Haojie Chen,Shiqi Tu,Hsin-Yi Huang,Yunjian Pan,Xiuqi Gui,Muyu Kuang,Xuxia Shen,Qiang Zheng,Yang Zhang,Chao Cheng,Hui Hong,Xiaoting Tao,Yizhou Peng,Xingxin Yao,Feilong Meng,Hongbin Ji,Zhen Shao,Yihua Sun

Journal of immunology research 2021:1057186 PubMed33506054

2021

Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Yanran Huang,Ming Wang,Yu Hong,Xiangting Bu,Ge Luan,Yang Wang,Ying Li,Hongfei Lou,Chengshuo Wang,Luo Zhang

Nature communications 11:991 PubMed32080185

2020

Tracing tumorigenesis in a solid tumor model at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha D Praktiknjo,Benedikt Obermayer,Qionghua Zhu,Liang Fang,Haiyue Liu,Hazel Quinn,Marlon Stoeckius,Christine Kocks,Walter Birchmeier,Nikolaus Rajewsky

Scientific reports 9:2827 PubMed30808940

2019

Chronic cerebral hypoperfusion shifts the equilibrium of amyloid β oligomers to aggregation-prone species with higher molecular weight.

Applications

Unspecified application

Species

Unspecified reactive species

Taro Bannai,Tatsuo Mano,Xigui Chen,Gaku Ohtomo,Ryo Ohtomo,Takeyuki Tsuchida,Kagari Koshi-Mano,Tadafumi Hashimoto,Hitoshi Okazawa,Takeshi Iwatsubo,Shoji Tsuji,Tatsushi Toda,Atsushi Iwata

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Clusterin antibody [EPR2911]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (AB92548)

Western blot - Anti-Clusterin antibody [EPR2911] (AB92548)

Western blot - Anti-Clusterin antibody [EPR2911] (AB92548)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise