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Rabbit Recombinant Monoclonal Clusterin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (AB184099), expandable thumbnail
  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (AB184099), expandable thumbnail
  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (AB184099), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (AB184099), expandable thumbnail
  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (AB184099), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins (By similarity). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed:14741101). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity (By similarity). Following stress, promotes apoptosis (PubMed:12551933). Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. Following ER stress, suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. When secreted, does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (By similarity). When secreted, acts as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (PubMed:11865066).

Alternative names

Apoj, Msgp-2, Clu, Clusterin, Apolipoprotein J, Clustrin, Sulfated glycoprotein 2, Apo-J, SGP-2

Recommended products

Rabbit Recombinant Monoclonal Clusterin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17538-101
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Clusterin beta chain also known as Apolipoprotein J is part of the clusterin protein complex that goes through heterodimerization to form a mature glycoprotein composed of alpha and beta chains. The estimated mass of the clusterin beta chain subunit is around 35-40 kDa. This protein is present in many tissues throughout the body with higher expression noted in the brain testes and kidneys as well as in blood plasma. It functions in molecular chaperoning and stabilizes numerous proteins and cells under stressful conditions.

Biological function summary

Clusterin beta chain engages in diverse functions related to cell apoptosis cell signaling and lipid transport. It is part of a secreted glycoprotein complex that includes an alpha chain. In the extracellular space it plays a significant role in inhibiting programmed cell death while also engaging in the regulation of immune responses. Its molecular chaperone capability safeguards cells from aggregation-prone stressors.

Pathways

Clusterin beta chain interacts with pathways associated with neuroprotection and immune regulation. It significantly participates in the apoptosis signaling pathway where it relates to other proteins such as Bax and Bcl-2 family members. Additionally its presence in the complement cascade relates it to complement component proteins such as C1q and C3 indicating its involvement in immune responses.

Associated diseases and disorders

Clusterin beta chain relates strongly to neurodegenerative diseases and some cancers. In Alzheimer's disease its interaction with amyloid-beta highlights its potential impact in amyloid plaque formation and clearance. It associates with amyloid precursor proteins in the disease mechanism. Furthermore in various cancer progressions its altered expression links to tumor suppression and metastasis. Proteins like p53 and Hsp70 display interactions with clusterin beta chain within these contexts influencing tumor cell survival and spread.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla than the cortex of rat adrenal gland (PMID: 16436671) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 5 seconds.

    Lane 3: 3 seconds.

    Lane 4: 1 minute.

    The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).

    All lanes: Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: Rat serum at 20 µg

    Lane 3: Mouse testis tissue lysate at 20 µg

    Lane 4: Rat testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa

    Observed band size: 33 kDa, 70 kDa

  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Blocking and dilution buffer: 5% NFDM/TBST .

    Exposure times.

    Lanes 1, 2 & 3: 3 minutes.

    Lane 4: 53 seconds.

    Lanes 5 & 6: 100 seconds.

    The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument

    All lanes: Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: Mouse adrenal gland tissue lysate at 20 µg

    Lane 3: Rat adrenal gland tissue lysate at 20 µg

    Lane 4: Rat lung tissue lysate at 20 µg

    Lane 5: Mouse stomach tissue lysate at 20 µg

    Lane 6: Rat stomach tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa

    Observed band size: 33 kDa, 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).

    All lanes: Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

    Lane 1: Wild-type mouse adrenal gland tissue lysate (male)

    Lane 2: Clusterin knockout adrenal gland tissue lysate (male)

    Lane 3: Wild-type mouse spleen tissue lysate (male)

    Lane 4: Clusterin knockout spleen tissue lysate (male)

    Lane 5: Wild-type mouse testis tissue lysate (male)

    Lane 6: Clusterin knockout testis tissue lysate (male)

    Lane 7: Wild-type mouse adrenal gland tissue lysate (female)

    Lane 8: Clusterin knockout adrenal gland tissue lysate (female)

    Lane 9: Wild-type mouse spleen tissue lysate (female)

    Lane 10: Clusterin knockout spleen tissue lysate (female)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded (A) Adrenal gland tissue from wild-type C57BL/6J mice (B) Adrenal gland tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Adrenal gland tissue from wild-type C57BL/6J mice and no staining on (B) Adrenal gland tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6J mice (B) Spleen tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6J mice and no staining on (B) Spleen tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.
    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

    Immunohistochemical analysis of paraffin-embedded (A) Testis tissue from wild-type C57BL/6J mice (B) Testis tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Testis tissue from wild-type C57BL/6J mice and no staining on (B) Testis tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).

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