AB235434

Anti-CLYBL/CLB antibody

Name: Anti-CLYBL/CLB antibody (ab235434) | Abcam
Brand: Abcam Limited
SKU: AB235434

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(2 Publications)

Rabbit Polyclonal CLYBL/CLB antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CLYBL.

View Alternative Names

CLB, CLYBL, (3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citrate lyase subunit beta-like protein, Malate synthase, Citrate lyase beta-like

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling CLYBL/CLB with ab235434 at 1/50 dilution, followed by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Antigen retrieval was performed using high pressure in a citrate buffer (pH 6.0). The section was blocked with 10% normal goat serum for 30 mins at 37°C, before being incubated with the primary antibody at 4°C overnight.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Paraffin-embedded human liver tissue stained for CLYBL/CLB using ab235434 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Paraffin-embedded human rectum tissue stained for CLYBL/CLB using ab235434 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Paraffin-embedded human kidney tissue stained for CLYBL/CLB using ab235434 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-CLYBL/CLB antibody (AB235434)

All lanes:

Western blot - Anti-CLYBL/CLB antibody (ab235434) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-CLYBL/CLB antibody (AB235434)

All lanes:

Western blot - Anti-CLYBL/CLB antibody (ab235434) at 1/1000 dilution

Lane 1:

Hela whole cell lysate

Lane 2:

Jurkat whole cell lysate

Lane 3:

THP-1 whole cell lysate

Lane 4:

HepG2 whole cell lysate

Lane 5:

NIH/3T3 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 37 kDa

Observed band size: 38 kDa

false

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CLYBL.

Q8N0X4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed : 29056341). Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway (PubMed : 29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed : 29056341). Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate (PubMed : 24334609, PubMed : 29056341). Also displays malyl-CoA thioesterase activity (PubMed : 29056341). Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate (PubMed : 24334609, PubMed : 29056341). Also has very weak citramalate synthase activity in vitro (PubMed : 24334609, PubMed : 29056341).

See full target information CLYBL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:415 PubMed40211376

2025

Acetylation suppresses breast cancer progression by sustaining CLYBL stability.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Deng,Chenglong Ma,Xingyu Chen,Ming Yi,Qianhua Cao,Ruocen Liao,Xingyu Lei,Longchang Bai,Bin Zhao,Yingnan Wang,Zhuoyang Shen,Liujing Wu,Chenfang Dong,Zhijun Dai

American journal of cancer research 14:1768-1783 PubMed38726285

2024

Roles of RNA mC modification patterns in prognosis and tumor microenvironment infiltration of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Cui,Cancan Luo,Lili Zhou,Tiantian Yu,Yongsheng Meng,Qianqian Yu,Zhixiang Lei,Ya Wang,Lijuan Peng,Qingqing Luo,Duozhuang Tang,Ruifang Sun,Li Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CLYBL/CLB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLYBL/CLB antibody (AB235434)

Western blot - Anti-CLYBL/CLB antibody (AB235434)

Western blot - Anti-CLYBL/CLB antibody (AB235434)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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