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AB230442

Anti-CMKLR1 antibody

0

(1 Review)

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(5 Publications)

Rabbit Polyclonal CMKLR1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CMKLR1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CHEMR23, DEZ, CMKLR1, Chemerin-like receptor 1, Chemokine-like receptor 1, G-protein coupled receptor ChemR23, G-protein coupled receptor DEZ

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMKLR1 antibody (AB230442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMKLR1 antibody (AB230442)

Formalin-fixed, paraffin-embedded human mesothelium tissue stained for CMKLR1 with ab230442 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMKLR1 antibody (AB230442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMKLR1 antibody (AB230442)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CMKLR1 with ab230442 at 1/100 dilution in immunohistochemical analysis. Incubated with the primary antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot - Anti-CMKLR1 antibody (AB230442)
  • WB

Supplier Data

Western blot - Anti-CMKLR1 antibody (AB230442)

All lanes:

Western blot - Anti-CMKLR1 antibody (ab230442) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

Mouse liver whole cell lysate

Lane 4:

Rat liver whole cell lysate

Predicted band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CMKLR1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q99788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "500-1000 µg/mL", "WB-species-notes": "<p></p>" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 initiates activation of G proteins G(i)/G(o) and beta-arrestin pathways inducing cellular responses via second messenger pathways such as intracellular calcium mobilization, phosphorylation of MAP kinases MAPK1/MAPK3 (ERK1/2), TYRO3, MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis (PubMed : 27716822). Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Positively regulates adipogenesis and adipocyte metabolism.. (Microbial infection) Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
See full target information CMKLR1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36359855

2022

Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Zhang,Qi Wu,Shuqi Zhu,Yibo Tang,Yanmin Chen,Danqing Chen,Zhaoxia Liang

Histology and histopathology 38:329-337 PubMed36156768

2022

Up-regulation of CMKLR1 in endometriosis and its relationship with inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Zhang,Yumei Ding,Junjie Li,Shan Su

General and comparative endocrinology 299:113584 PubMed32827511

2020

Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: A possible involvement in the fattening and the onset of puberty.

Applications

Unspecified application

Species

Unspecified reactive species

Alix Barbe,Patrycja Kurowska,Ewa Mlyczyńska,Christelle Ramé,Christophe Staub,Eric Venturi,Yvon Billon,Agnieszka Rak,Joëlle Dupont

International journal of molecular sciences 21: PubMed32717877

2020

The In Vitro Effect of Prostaglandin E and F on the Chemerin System in the Porcine Endometrium during Gestation.

Applications

Unspecified application

Species

Unspecified reactive species

Kamil Dobrzyn,Marta Kiezun,Ewa Zaobidna,Katarzyna Kisielewska,Edyta Rytelewska,Marlena Gudelska,Grzegorz Kopij,Kinga Bors,Karolina Szymanska,Barbara Kaminska,Tadeusz Kaminski,Nina Smolinska

International journal of molecular sciences 20: PubMed31404950

2019

Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Smolinska,Marta Kiezun,Kamil Dobrzyn,Edyta Rytelewska,Katarzyna Kisielewska,Marlena Gudelska,Ewa Zaobidna,Krystyna Bogus-Nowakowska,Joanna Wyrebek,Kinga Bors,Grzegorz Kopij,Barbara Kaminska,Tadeusz Kaminski
View all publications
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