Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free

10 product images

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  Western blot - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  CMKLR1 Western blot staining using rabbit Anti-CMKLR1 antibody

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregation.
  5.5 seconds
  Exposure time:

  All lanes: Western blot - Anti-CMKLR1 antibody [EPR26501-70] (Anti-CMKLR1 antibody [EPR26501-70] ab306554) at 1/1000 dilution

  Lane 1: Human tonsil tissue lysate 20 μg

  Lane 2: Human placenta tissue lysate 20 μg

  Lane 3: Mouse spleen tissue lysate 20 μg

  Lane 4: Rat spleen tissue lysate 20 μg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 42 kDa

  Exposure time: 5.5s

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  Flow Cytometry - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 3 days cells labelling CMKLR1 with Anti-CMKLR1 antibody [EPR26501-70] ab306554 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor? 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Cells were stained with rabbit IgG or Anti-CMKLR1 antibody [EPR26501-70] ab306554. Then stained with anti-CD123 conjugated to FITC.

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  Flow Cytometry - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 3 days cells labelling CMKLR1 with Anti-CMKLR1 antibody [EPR26501-70] ab306554 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor? 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Cells were stained with rabbit IgG or Anti-CMKLR1 antibody [EPR26501-70] ab306554. Then stained with anti-HLA-DR conjugated to PE.

• 

  Flow Cytometry - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 3 days cells labelling CMKLR1 with Anti-CMKLR1 antibody [EPR26501-70] ab306554 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor? 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable HLA-DR and CD123 double positive cells.Cells were stained with rabbit IgG or Anti-CMKLR1 antibody [EPR26501-70] ab306554. Then stained with anti-CD123 conjugated to FITC and anti-HLA-DR conjugated to PE.

• 

  Western blot - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregation.
  5.5 seconds
  Exposure time:

  All lanes: Western blot - Anti-CMKLR1 antibody [EPR26501-70] (Anti-CMKLR1 antibody [EPR26501-70] ab306554) at 1/1000 dilution

  Lane 1: Human fat tissue lysate 20 μg

  Lane 2: Mouse white fat tissue lysate 20 μg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 42 kDa

  Exposure time: 5.5s

• 

  Western blot - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregation.
  5.5 seconds
  Exposure time:

  All lanes: Western blot - Anti-CMKLR1 antibody [EPR26501-70] (Anti-CMKLR1 antibody [EPR26501-70] ab306554) at 1/1000 dilution

  Lane 1: Ea.Hy926 (human somatic cell hybrid endothelial cell) whole cell lysate 20 μg

  Lane 2: HUVEC (human umbilical vein endothelial cell) whole cell lysate 20 μg

  Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20 μg

  Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

  Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 42 kDa

  Exposure time: 5.5s

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  Immunocytochemistry/ Immunofluorescence - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling CMKLR1 with Anti-CMKLR1 antibody [EPR26501-70] ab306554 at 1/100 (5.25 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in Neuro-2a cell line Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) cells labeling CMKLR1 with Anti-CMKLR1 antibody [EPR26501-70] ab306554 at 1/100 (5.25 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in HL-60 cells treated with PMA (50 ng/ml) for 72 h, then add MG-132 (10 uM) for 4 h, and no staining under only MG-132 (10 uM) for 4 h treatment. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EA.hy926 (human somatic cell hybrid endothelial) cells labeling CMKLR1 with Anti-CMKLR1 antibody [EPR26501-70] ab306554 at 1/100 (5.25 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in EA.hy926 cell line Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Western blot - Anti-CMKLR1 antibody [EPR26501-70] - BSA and Azide free (ab306555)

  This data was developed using Anti-CMKLR1 antibody [EPR26501-70] ab306554, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Samples are non-boiled as boiling may cause protein aggregation.
  
  The expression of Chemokine-like receptor 1 is upregulated in response to PMA treatment (PMID: 19906641).
  
  
  3 seconds
  Exposure time:

  All lanes: Western blot - Anti-CMKLR1 antibody [EPR26501-70] (Anti-CMKLR1 antibody [EPR26501-70] ab306554) at 1/1000 dilution

  Lane 1: HL-60 (human acute promyelocytic leukemia promyeloblast) treated with 10 ?M MG-132 for 4h whole cell lysate 20 µg

  Lane 2: HL-60 treated with /ml PMA for 72h, 10 ?M MG-132 was then added for additional 4h whole cell lysate 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Observed band size: 42 kDa

  Exposure time: 3s