Anti-CMTM4 antibody [EPR27409-50]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling CMTM4 with ab307501 at 1/2000 dilution (0.227 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human stomach. The section was incubated with ab307501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

CMTM4 was immunoprecipitated from 0.35 mg NCI-H1975 (Human adenocarcinoma lung epithelial cell) whole cell lysate 10 µg with ab307501 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307501 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NCI-H1975 whole cell lysate 10 µg Lane 2 : ab307501 IP in NCI-H1975 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307501 in NCI-H1975 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-CMTM4 antibody [EPR27409-50] (ab307501) at 1/30 dilution

All lanes:

NCI-H1975 (Human adenocarcinoma lung epithelial cell)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 19 kDa,22 kDa

false

Exposure time: 15s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CMTM4 with ab307501 at 1/2000 dilution (0.227 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat stomach. The section was incubated with ab307501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Immunohistochemical analysis of paraffin-embedded mouse pancreatic tumor tissue labeling CMTM4 with ab307501 at 1/2000 dilution (0.227 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pancreatic tumor. The section was incubated with ab307501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling CMTM4 with ab307501 at 1/2000 dilution (0.227 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse stomach. The section was incubated with ab307501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

CMTM4 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg with ab307501 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307501 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 whole cell lysate 10 µg Lane 2 : ab307501 IP in NIH/3T3 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307501 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 8 seconds.

All lanes:

Immunoprecipitation - Anti-CMTM4 antibody [EPR27409-50] (ab307501) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 19 kDa,22 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : human skeletal muscle (PMID : 20213316) . Exposure time : 180 seconds.

All lanes:

Western blot - Anti-CMTM4 antibody [EPR27409-50] (ab307501) at 1/1000 dilution

Lane 1:

Human testis membrane tissue lysate at 20 µg

Lane 2:

Human stomach tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 19 kDa,22 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure times : Lanes 1 : 114 seconds Lane 2 : 125 seconds

All lanes:

Western blot - Anti-CMTM4 antibody [EPR27409-50] (ab307501) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 19 kDa,22 kDa

false

• WB

Supplier Data

Western blot - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HeLa (PMID : 20213316).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-CMTM4 antibody [EPR27409-50] (ab307501) at 1/1000 dilution

Lane 1:

NCI-H1975 (human adenocarcinoma lung epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 19 kDa,22 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-CMTM4 antibody [EPR27409-50] (ab307501) at 1/1000 dilution

All lanes:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 22 kDa

false

Exposure time: 180s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling CMTM4 with ab307501 at 1/2000 dilution (0.227 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse skeletal muscle. The section was incubated with ab307501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM4 antibody [EPR27409-50] (AB307501)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling CMTM4 with ab307501 at 1/2000 dilution (0.227 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human skeletal muscle. The section was incubated with ab307501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.