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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal CMTM6 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Tested | Expected |
Mouse | Tested | Not recommended | Expected | Tested | Tested |
Rat | Tested | Not recommended | Expected | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Rat | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.
CKLF-like MARVEL transmembrane domain-containing protein 6, Chemokine-like factor superfamily member 6, CMTM6, CKLFSF6
Rabbit Recombinant Monoclonal CMTM6 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
CKLF-like MARVEL transmembrane domain-containing protein 6, Chemokine-like factor superfamily member 6, CMTM6, CKLFSF6
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR23015-45
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-2: 3 minutes
Lane 3: 48 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28813417).
All lanes: Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/1000 dilution
Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: Human tonsil tissue lysate at 20 µg
Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human pancreatic cancer (PMID/ 28813417) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human thyroid cancer (PMID: 28813410) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
ab264067 at 1/30 dilution immunoprecipitating CMTM6 in rat spleen tissue lysate. Western blot was performed from the immunoprecipitate using ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Rat spleen tissue lysate 10 µg
Lane 2: ab264067 IP in rat spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264067 in rat spleen tissue lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 6 seconds.
Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)
All lanes: Mouse spleen tissue lysate at 10 µg
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution
Exposure time: 6s
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
ab181602 was used as GAPDH loading control.
We recommend not to boil the samples after lysis to get desired WB bands.
All lanes: Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/10000 dilution
Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg
Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg
Lane 3: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg
Lane 4: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg
Lane 5: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled at 20 µg
Lane 6: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 7s
Exposure time:
Lane 1-2: 15 seconds
Lane 3-5: 10 seconds
Blocking/Diluting buffer and concentration 5% NFDM/TBST
Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1/200000) (36KDa).
Low expression: skeletal muscle.
This blot was developed using a high sensitivity ECL substrate.
Samples are non-boiled as boiling may cause protein aggregates.
Lanes 1 - 5: Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/1000 dilution
Lanes 1 - 5: Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
Lane 5: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CMTM6 with ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Positive staining on mouse testis.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with ab264067 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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