Anti-CMTM6 antibody [EPR23015-45]
- BOND RX™ Validated
- RabMAb
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(8 Publications)
Rabbit Recombinant Monoclonal CMTM6 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
View Alternative Names
CKLFSF6, CMTM6, CKLF-like MARVEL transmembrane domain-containing protein 6, Chemokine-like factor superfamily member 6
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human thyroid cancer (PMID : 28813410) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human pancreatic cancer (PMID/ 28813417) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with ab264067 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CMTM6 with ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on mouse testis. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CMTM6 with ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Low expression : nearly no staining on mouse skeletal muscle. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
- IP
Lab
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
ab264067 at 1/30 dilution immunoprecipitating CMTM6 in mouse spleen tissue lysate. Western blot was performed from the immunoprecipitate using ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : Mouse spleen tissue lysate 10 µg Lane 2 : ab264067 IP in mouse spleen tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab264067 in mouse spleen tissue lysate. Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : 8 seconds.
Lane 2:
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/30 dilution
Lane 2:
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076'>ab264076</a>)
All lanes:
Mouse spleen tissue lysate at 10 µg
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 8s
- IP
Lab
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
ab264067 at 1/30 dilution immunoprecipitating CMTM6 in rat spleen tissue lysate. Western blot was performed from the immunoprecipitate using ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : Rat spleen tissue lysate 10 µg Lane 2 : ab264067 IP in rat spleen tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab264067 in rat spleen tissue lysate. Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : 6 seconds.
Lane 2:
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/30 dilution
Lane 2:
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076'>ab264076</a>)
All lanes:
Mouse spleen tissue lysate at 10 µg
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 6s
- WB
Lab
Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as GAPDH loading control. We recommend not to boil the samples after lysis to get desired WB bands.
All lanes:
Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/10000 dilution
Lane 1:
MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg
Lane 2:
MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg
Lane 3:
BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg
Lane 4:
BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg
Lane 5:
Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled at 20 µg
Lane 6:
Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
false
Exposure time: 7s
- WB
Unknown
Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure times.
Lanes 1-2 : 3 minutes
Lane 3 : 48 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28813417).
All lanes:
Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/1000 dilution
Lane 1:
MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2:
Human tonsil tissue lysate at 20 µg
Lane 3:
BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
false
- WB
Lab
Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
Exposure time : Lane 1-2 : 15 seconds Lane 3-5 : 10 seconds Blocking/Diluting buffer and concentration 5% NFDM/TBST Loading control : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1/200000) (36KDa). Low expression : skeletal muscle. This blot was developed using a high sensitivity ECL substrate. Samples are non-boiled as boiling may cause protein aggregates.
Lanes 1 - 5:
Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/1000 dilution
Lanes 1 - 5:
Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076'>ab264076</a>)
Lane 1:
Mouse spleen tissue lysate at 20 µg
Lane 2:
Mouse skeletal muscle tissue lysate at 20 µg
Lane 3:
Rat spleen tissue lysate at 20 µg
Lane 4:
Rat skeletal muscle tissue lysate at 20 µg
Lane 5:
Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
false
Related conjugates and formulations (10)
-
Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free
-
660 APC
APC Anti-CMTM6 antibody [EPR23015-45]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-CMTM6 antibody [EPR23015-45]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-CMTM6 antibody [EPR23015-45]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-CMTM6 antibody [EPR23015-45]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-CMTM6 antibody [EPR23015-45]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-CMTM6 antibody [EPR23015-45]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-CMTM6 antibody [EPR23015-45]
-
HRP Anti-CMTM6 antibody [EPR23015-45]
-
578 PE
PE Anti-CMTM6 antibody [EPR23015-45]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (8)
Recent publications for all applications. Explore the full list and refine your search
Journal of experimental & clinical cancer research : CR 44:257 PubMed40877908
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 25: PubMed38542462
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of clinical and experimental pathology 16:368-377 PubMed38188349
2024
Applications
Unspecified application
Species
Unspecified reactive species
Journal for immunotherapy of cancer 11: PubMed37349124
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cancer immunology research 11:241-260 PubMed36484740
2022
Applications
Unspecified application
Species
Unspecified reactive species
Acta pharmacologica Sinica 44:1095-1104 PubMed36418428
2022
Applications
Unspecified application
Species
Unspecified reactive species
BioMed research international 2022:8118909 PubMed35845949
2022
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in oncology 11:757919 PubMed34760703
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com