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AB264067

Anti-CMTM6 antibody [EPR23015-45]

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(8 Publications)

Rabbit Recombinant Monoclonal CMTM6 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

CKLFSF6, CMTM6, CKLF-like MARVEL transmembrane domain-containing protein 6, Chemokine-like factor superfamily member 6

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human thyroid cancer (PMID : 28813410) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human pancreatic cancer (PMID/ 28813417) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with ab264067 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CMTM6 with ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on mouse testis. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CMTM6 with ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Low expression : nearly no staining on mouse skeletal muscle. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IP

Lab

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

ab264067 at 1/30 dilution immunoprecipitating CMTM6 in mouse spleen tissue lysate. Western blot was performed from the immunoprecipitate using ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : Mouse spleen tissue lysate 10 µg Lane 2 : ab264067 IP in mouse spleen tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab264067 in mouse spleen tissue lysate. Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : 8 seconds.

Lane 2:

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076'>ab264076</a>)

All lanes:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • IP

Lab

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

ab264067 at 1/30 dilution immunoprecipitating CMTM6 in rat spleen tissue lysate. Western blot was performed from the immunoprecipitate using ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : Rat spleen tissue lysate 10 µg Lane 2 : ab264067 IP in rat spleen tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab264067 in rat spleen tissue lysate. Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : 6 seconds.

Lane 2:

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076'>ab264076</a>)

All lanes:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • WB

Lab

Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as GAPDH loading control. We recommend not to boil the samples after lysis to get desired WB bands.

All lanes:

Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/10000 dilution

Lane 1:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg

Lane 2:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg

Lane 3:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg

Lane 4:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg

Lane 5:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled at 20 µg

Lane 6:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Exposure time: 7s

Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • WB

Unknown

Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1-2 : 3 minutes

Lane 3 : 48 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28813417).

All lanes:

Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/1000 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Lane 3:

BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)
  • WB

Lab

Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067)

Exposure time : Lane 1-2 : 15 seconds Lane 3-5 : 10 seconds Blocking/Diluting buffer and concentration 5% NFDM/TBST Loading control : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1/200000) (36KDa). Low expression : skeletal muscle. This blot was developed using a high sensitivity ECL substrate. Samples are non-boiled as boiling may cause protein aggregates.

Lanes 1 - 5:

Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067) at 1/1000 dilution

Lanes 1 - 5:

Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076'>ab264076</a>)

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Lane 5:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • Carrier free

    Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free

  • 660 APC

    APC Anti-CMTM6 antibody [EPR23015-45]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CMTM6 antibody [EPR23015-45]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CMTM6 antibody [EPR23015-45]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CMTM6 antibody [EPR23015-45]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CMTM6 antibody [EPR23015-45]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CMTM6 antibody [EPR23015-45]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CMTM6 antibody [EPR23015-45]

  • HRP

    HRP Anti-CMTM6 antibody [EPR23015-45]

  • 578 PE

    PE Anti-CMTM6 antibody [EPR23015-45]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23015-45

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt (Intra), WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.
See full target information CMTM6

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:257 PubMed40877908

2025

TRAP1 expression elicits pro-tumoral functions in macrophages associated to malignant peripheral nerve sheath tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Scantamburlo,Alessia Rubini,Margherita Toffanin,Maria Egle Castorina,Francesco Ciscato,Sofia Tomasoni,Paolo Finotti,Ranieri Verin,Valentina Zappulli,Marco Fantuz,Camilla Bean,Andrea Rasola,Ionica Masgras

International journal of molecular sciences 25: PubMed38542462

2024

Differential Expression of CKLF-like MARVEL Transmembrane Domain-Containing Protein 6 and Programmed Cell Death Ligand 1 as Prognostic Biomarkers in Upper Tract Urothelial Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Said Kdimati,Clemens Christoph,Änne Glass,Nadja Engel,Desiree-Louise Dräger,Claudia Maletzki,Anne-Sophie Becker,Annette Zimpfer

International journal of clinical and experimental pathology 16:368-377 PubMed38188349

2024

Clinicopathologic and prognostic significance of CMTM6 and PD-L1 expression in cervical squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Ma,Shuai Shi,Zhihong Ma,Jie Sun,Xiaoyun Liu,Shulei Niu,Honggang Liu,Zhigang Zhang

Journal for immunotherapy of cancer 11: PubMed37349124

2023

circ-0000512 inhibits PD-L1 ubiquitination through sponging miR-622/CMTM6 axis to promote triple-negative breast cancer and immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Feng Dong,Fang-Fang Chen,Yang-Fan Fan,Kun Zhang,Hui-Hui Chen

Cancer immunology research 11:241-260 PubMed36484740

2022

Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1-Independent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yiru Long,Runqiu Chen,Xiaolu Yu,Yongliang Tong,Xionghua Peng,Fanglin Li,Chao Hu,Jianhua Sun,Likun Gong

Acta pharmacologica Sinica 44:1095-1104 PubMed36418428

2022

Construction of stable membranal CMTM6-PD-L1 full-length complex to evaluate the PD-1/PD-L1-CMTM6 interaction and develop anti-tumor anti-CMTM6 nanobody.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Min Jia,Yi-Ru Long,Xiao-Lu Yu,Run-Qiu Chen,Li-Kun Gong,Yong Geng

BioMed research international 2022:8118909 PubMed35845949

2022

Expression and Clinical Significance of and in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Shi,Hong-Yan Ma,Yin-Zhou Sang,Ying-Bo Ju,Xiao-Yun Liu,Zhi-Gang Zhang

Frontiers in oncology 11:757919 PubMed34760703

2021

Computational Recognition and Clinical Verification of TGF-β-Derived miRNA Signature With Potential Implications in Prognosis and Immunotherapy of Intrahepatic Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zaoqu Liu,Siyuan Weng,Hui Xu,Libo Wang,Long Liu,Yuyuan Zhang,ChunGuang Guo,Qin Dang,Zhe Xing,Taoyuan Lu,Xinwei Han
View all publications

Product promise

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