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Rabbit Recombinant Monoclonal CMTM6 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PIP
Human
Tested
Not recommended
Tested
Tested
Expected
Mouse
Tested
Not recommended
Expected
Tested
Tested
Rat
Tested
Not recommended
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Species

Rat

Dilution info

1/30

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.

Alternative names

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Rabbit Recombinant Monoclonal CMTM6 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23015-45

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-2: 3 minutes

    Lane 3: 48 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28813417).

    All lanes: Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/1000 dilution

    Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: Human tonsil tissue lysate at 20 µg

    Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Predicted band size: 20 kDa

    Observed band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human pancreatic cancer (PMID/ 28813417) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human thyroid cancer (PMID: 28813410) The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    ab264067 at 1/30 dilution immunoprecipitating CMTM6 in rat spleen tissue lysate. Western blot was performed from the immunoprecipitate using ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Rat spleen tissue lysate 10 µg

    Lane 2: ab264067 IP in rat spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264067 in rat spleen tissue lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 6 seconds.

    Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/30 dilution

    Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)

    All lanes: Mouse spleen tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Exposure time: 6s

  • Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    ab181602 was used as GAPDH loading control.

    We recommend not to boil the samples after lysis to get desired WB bands.

    All lanes: Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/10000 dilution

    Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg

    Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg

    Lane 3: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg

    Lane 4: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg

    Lane 5: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled at 20 µg

    Lane 6: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 20 kDa

    Observed band size: 20 kDa

    Exposure time: 7s

  • Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Western blot - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Exposure time:
    Lane 1-2: 15 seconds
    Lane 3-5: 10 seconds

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1/200000) (36KDa).

    Low expression: skeletal muscle.
    This blot was developed using a high sensitivity ECL substrate.
    Samples are non-boiled as boiling may cause protein aggregates.

    Lanes 1 - 5: Western blot - Anti-CMTM6 antibody [EPR23015-45] (AB264067) at 1/1000 dilution

    Lanes 1 - 5: Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 3: Rat spleen tissue lysate at 20 µg

    Lane 4: Rat skeletal muscle tissue lysate at 20 µg

    Lane 5: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CMTM6 with ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
    Positive staining on mouse testis.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] (ab264067), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] (ab264067)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with ab264067 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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