Rabbit Recombinant Monoclonal CMTM6 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111033.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134192.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99144.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)")
![Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--western-blot-img436125.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436122.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (AB264076)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested | Expected |
| Mouse | Tested | Not recommended | Expected | Tested | Tested |
| Rat | Tested | Not recommended | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
8 products for Alternative Version
Target data
Function
Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.
Alternative names
CKLFSF6, CMTM6, CKLF-like MARVEL transmembrane domain-containing protein 6, Chemokine-like factor superfamily member 6
Recommended products
Rabbit Recombinant Monoclonal CMTM6 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P, IP and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23015-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab264076 is the carrier-free version of Anti-CMTM6 antibody [EPR23015-45] ab264067.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111033.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer tissue labeling CMTM6 with Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human pancreatic cancer (PMID: 28813417) The section was incubated with Anti-CMTM6 antibody [EPR23015-45] ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134192.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling CMTM6 with Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human thyroid cancer (PMID: 28813410) The section was incubated with Anti-CMTM6 antibody [EPR23015-45] ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99144.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CMTM6 with Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/2000 dilution (0.24 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with Anti-CMTM6 antibody [EPR23015-45] ab264067 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).
![Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--western-blot-img436125.jpg "Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).
Exposure time:
Lane 1-2: 15 seconds
Lane 3-5: 10 seconds
Blocking/Diluting buffer and concentration 5% NFDM/TBST
Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1/200000) (36KDa).
Low expression: skeletal muscle.
This blot was developed using a high sensitivity ECL substrate.
Samples are non-boiled as boiling may cause protein aggregates.Lanes 1 - 5: Western blot - Anti-CMTM6 antibody [EPR23015-45] (Anti-CMTM6 antibody [EPR23015-45] ab264067) at 1/1000 dilution
Lanes 1 - 5: Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
Lane 5: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436122.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CMTM6 with Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Positive staining on mouse testis.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).![Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--flow-cytometry-intracellular-img413771.jpg "Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling CMTM6 with Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436121.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CMTM6 with Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/2000 dilution, incubated for 30 mins at room temperature, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Low expression: nearly no staining on mouse skeletal muscle.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).![Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--western-blot-img415956.jpg "Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.
We recommend not to boil the samples after lysis to get desired WB bands.All lanes: Western blot - Anti-CMTM6 antibody [EPR23015-45] (Anti-CMTM6 antibody [EPR23015-45] ab264067) at 1/10000 dilution
Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg
Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg
Lane 3: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg
Lane 4: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg
Lane 5: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled at 20 µg
Lane 6: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 7s
![Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunoprecipitation-img436123.jpg "Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/30 dilution immunoprecipitating CMTM6 in mouse spleen tissue lysate. Western blot was performed from the immunoprecipitate using Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10 µg
Lane 2: Anti-CMTM6 antibody [EPR23015-45] ab264067 IP in mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CMTM6 antibody [EPR23015-45] ab264067 in mouse spleen tissue lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (Anti-CMTM6 antibody [EPR23015-45] ab264067) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
All lanes: Mouse spleen tissue lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
![Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunoprecipitation-img436124.jpg "Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/30 dilution immunoprecipitating CMTM6 in rat spleen tissue lysate. Western blot was performed from the immunoprecipitate using Anti-CMTM6 antibody [EPR23015-45] ab264067 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: Rouse spleen tissue lysate 10 µg
Lane 2: Anti-CMTM6 antibody [EPR23015-45] ab264067 IP in rat spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CMTM6 antibody [EPR23015-45] ab264067 in rat spleen tissue lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 6 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CMTM6 antibody [EPR23015-45] ab264067).Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] (Anti-CMTM6 antibody [EPR23015-45] ab264067) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)
All lanes: Mouse spleen tissue lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 6s
![Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--flow-cytometry-intracellular-img413771.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436121.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
![Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--western-blot-img415956.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
![Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076), expandable thumbnail](https://content.abcam.com/products/images/cmtm6-antibody-epr23015-45-bsa-and-azide-free-ab264076--immunoprecipitation-img436123.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CMTM6 antibody [EPR23015-45] - BSA and Azide free (ab264076)")
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