Rabbit Polyclonal CNBP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CNBP aa 1-100.
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Publications
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- Journal of oncology 2022:26741632022CircFMN2 Boosts Sorafenib Resistance in Hepatocellular Carcinoma Cells via Upregulating CNBP by Restraining Ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Fan,Xiaoli Zhu,Qi Zhou,Weidong WangPubMed 35909906
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CNBP aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P62633
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04-0.4 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
Target data
Function
Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression (PubMed:2562787). Has a role as transactivator of the Myc promoter (By similarity). Binds single-stranded RNA in a sequence-specific manner (By similarity). Isoform 1. Binds G-rich elements in target mRNA coding sequences (PubMed:28329689). Prevents G-quadruplex structure formation in vitro, suggesting a role in supporting translation by resolving stable structures on mRNAs (PubMed:28329689). Isoform 2. Binds to RNA. Isoform 4. Binds to RNA. Isoform 5. Binds to RNA. Isoform 6. Binds to RNA. Isoform 8. Binds to RNA.
Alternative names
RNF163, ZNF9, CNBP, CCHC-type zinc finger nucleic acid binding protein, Cellular nucleic acid-binding protein, Zinc finger protein 9
Recommended products
Rabbit Polyclonal CNBP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CNBP aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CNBP aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P62633
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-CNBP antibody (ab272676)
All lanes: Western blot - Anti-CNBP antibody (ab272676) at 0.4 µg/mL
Lane 1: RT4 cells (Human urinary bladder cancer cell line)
Lane 2: U-251 MG cells (Human brain glioma cell line)
Lane 3: Human plasma
Lane 4: Human liver tissue
Lane 5: Human tonsil tissue
Predicted band size: 19 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab272676)
PFA fixed, Triton X-100 permeablized SH-SY5Y cells (human neuroblastoma cell line from bone marrow) labeling CNBP (green) using ab272676 at 2 ug/ml in ICC/IF analysis.
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