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AB48027

Anti-CNBP antibody

5

(3 Reviews)

|

(5 Publications)

Goat Polyclonal CNBP antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CNBP aa 150 to C-terminus.

View Alternative Names

RNF163, ZNF9, CNBP, CCHC-type zinc finger nucleic acid binding protein, Cellular nucleic acid-binding protein, Zinc finger protein 9

6 Images
Flow Cytometry - Anti-CNBP antibody (AB48027)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CNBP antibody (AB48027)

Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (AB48027)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (AB48027)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (AB48027)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (AB48027)

Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western blot - Anti-CNBP antibody (AB48027)
  • WB

Supplier Data

Western blot - Anti-CNBP antibody (AB48027)

Lysate in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-CNBP antibody (ab48027) at 1 µg/mL

Lane 1:

Daudi cell lysate at 35 µg

Lane 2:

MOLT-4 cell lysate at 35 µg

Lane 3:

MCF7 cell lysate at 35 µg

Lane 4:

Neuro2a cell lysate at 35 µg

false

Western blot - Anti-CNBP antibody (AB48027)
  • WB

Supplier Data

Western blot - Anti-CNBP antibody (AB48027)

Lysate in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-CNBP antibody (ab48027) at 1 µg/mL

All lanes:

Human Skeletal Muscle lysate at 35 µg

false

Western blot - Anti-CNBP antibody (AB48027)
  • WB

AbReview28292****

Western blot - Anti-CNBP antibody (AB48027)

All lanes:

Western blot - Anti-CNBP antibody (ab48027) at 1 µg/mL

Lane 1:

Whole cell lysate prepared from 9L cells at 20 µg

Lane 2:

Whole cell lysate prepared from PC12 cells at 20 µg

Lane 3:

Whole cell lysate prepared from RIE-1 cells at 20 µg

Secondary

All lanes:

HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa,65 kDa

true

Exposure time: 6min

Image courtesy of an anonymous Abreview.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human CNBP aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62633

Specificity

No crossreaction expected with CCHC domain containing 13.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression (PubMed : 2562787). Has a role as transactivator of the Myc promoter (By similarity). Binds single-stranded RNA in a sequence-specific manner (By similarity).. Isoform 1. Binds G-rich elements in target mRNA coding sequences (PubMed : 28329689). Prevents G-quadruplex structure formation in vitro, suggesting a role in supporting translation by resolving stable structures on mRNAs (PubMed : 28329689).. Isoform 2. Binds to RNA.. Isoform 4. Binds to RNA.. Isoform 5. Binds to RNA.. Isoform 6. Binds to RNA.. Isoform 8. Binds to RNA.
See full target information CNBP
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 13:e1235 PubMed37186134

2023

Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity.

Applications

Unspecified application

Species

Unspecified reactive species

Anpei Hu,Guo Chen,Banghe Bao,Yanhua Guo,Dan Li,Xiaojing Wang,Jianqun Wang,Qilan Li,Yi Zhou,Haiyang Gao,Jiyu Song,Xinyi Du,Liduan Zheng,Qiangsong Tong

International journal of biological sciences 18:1491-1507 PubMed35280687

2022

Circular RNA circDVL1 inhibits clear cell renal cell carcinoma progression through the miR-412-3p/PCDH7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yunjing Zhang,Xinwan Su,Qiongzi Qiu,Yuan Yuan,Chunhua Weng,Sailan Zou,Yan Tian,Weidong Han,Pengyuan Liu,Xingyi Guo,Jianhua Mao,Xianghui Fu,Ping Wang,Weiqiang Lin

eLife 10: PubMed34517941

2021

Translational control of polyamine metabolism by CNBP is required for locomotor function.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Coni,Federica A Falconio,Marta Marzullo,Marzia Munafò,Benedetta Zuliani,Federica Mosti,Alessandro Fatica,Zaira Ianniello,Rosa Bordone,Alberto Macone,Enzo Agostinelli,Alessia Perna,Tanja Matkovic,Stephan Sigrist,Gabriella Silvestri,Gianluca Canettieri,Laura Ciapponi

Molecular cancer 18:158 PubMed31718709

2019

Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Anpei Hu,Dan Li,Jianqun Wang,Yanhua Guo,Yang Liu,Hongjun Li,Yajun Chen,Xiaojing Wang,Kai Huang,Liduan Zheng,Qiangsong Tong

Cell cycle (Georgetown, Tex.) 13:434-9 PubMed24275942

2013

CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Antonucci,Davide D'Amico,Laura Di Magno,Sonia Coni,Lucia Di Marcotullio,Beatrice Cardinali,Alberto Gulino,Laura Ciapponi,Gianluca Canettieri
View all publications
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