Goat Polyclonal CNBP antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CNBP aa 150 to C-terminus.
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Publications
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- International journal of biological sciences 18:1491-15072022Circular RNA circDVL1 inhibits clear cell renal cell carcinoma progression through the miR-412-3p/PCDH7 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Wang,Yunjing Zhang,Xinwan Su,Qiongzi Qiu,Yuan Yuan,Chunhua Weng,Sailan Zou,Yan Tian,Weidong Han,Pengyuan Liu,Xingyi Guo,Jianhua Mao,Xianghui Fu,Ping Wang,Weiqiang LinPubMed 35280687
- eLife 10:2021Translational control of polyamine metabolism by CNBP is required for locomotor function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonia Coni,Federica A Falconio,Marta Marzullo,Marzia Munafò,Benedetta Zuliani,Federica Mosti,Alessandro Fatica,Zaira Ianniello,Rosa Bordone,Alberto Macone,Enzo Agostinelli,Alessia Perna,Tanja Matkovic,Stephan Sigrist,Gabriella Silvestri,Gianluca Canettieri,Laura CiapponiPubMed 34517941
- Molecular cancer 18:1582019Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Yang et. al.PubMed 31718709
- Cell cycle (Georgetown, Tex.) 13:434-92013CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Antonucci,Davide D'Amico,Laura Di Magno,Sonia Coni,Lucia Di Marcotullio,Beatrice Cardinali,Alberto Gulino,Laura Ciapponi,Gianluca CanettieriPubMed 24275942
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CNBP aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P62633
Reactivity data
Select an application| WB | Flow Cyt | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Mouse | Dilution info 0.30000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey | Dilution info - | Notes - |
Target data
Function
Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression (PubMed:2562787). Has a role as transactivator of the Myc promoter (By similarity). Binds single-stranded RNA in a sequence-specific manner (By similarity). Isoform 1. Binds G-rich elements in target mRNA coding sequences (PubMed:28329689). Prevents G-quadruplex structure formation in vitro, suggesting a role in supporting translation by resolving stable structures on mRNAs (PubMed:28329689). Isoform 2. Binds to RNA. Isoform 4. Binds to RNA. Isoform 5. Binds to RNA. Isoform 6. Binds to RNA. Isoform 8. Binds to RNA.
Alternative names
RNF163, ZNF9, CNBP, CCHC-type zinc finger nucleic acid binding protein, Cellular nucleic acid-binding protein, Zinc finger protein 9
Recommended products
Goat Polyclonal CNBP antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CNBP aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CNBP aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P62633
- Purification technique
- Affinity purification Immunogen
- Specificity
No crossreaction expected with CCHC domain containing 13.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
Image courtesy of an anonymous customer review.Western blot - Anti-CNBP antibody (ab48027)
All lanes: Western blot - Anti-CNBP antibody (ab48027) at 1 µg/mL
Lane 1: Whole cell lysate prepared from 9L cells at 20 µg
Lane 2: Whole cell lysate prepared from PC12 cells at 20 µg
Lane 3: Whole cell lysate prepared from RIE-1 cells at 20 µg
SecondaryAll lanes: HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa, 65 kDa
Exposure time: 6min
Western blot - Anti-CNBP antibody (ab48027)
Lysate in RIPA buffer. Detected by chemiluminescence.
All lanes: Western blot - Anti-CNBP antibody (ab48027) at 1 µg/mL
All lanes: Human Skeletal Muscle lysate at 35 µg
Western blot - Anti-CNBP antibody (ab48027)
Lysate in RIPA buffer. Detected by chemiluminescence.
All lanes: Western blot - Anti-CNBP antibody (ab48027) at 1 µg/mL
Lane 1: Daudi cell lysate at 35 µg
Lane 2: MOLT-4 cell lysate at 35 µg
Lane 3: MCF7 cell lysate at 35 µg
Lane 4: Neuro2a cell lysate at 35 µg
Flow Cytometry - Anti-CNBP antibody (ab48027)
Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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