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Mouse Recombinant Monoclonal CNGA1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (AB255766), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (AB255766), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (AB255766), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (AB255766), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-Fr
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1.01 µg/mL
Notes

-

Species
Rat
Dilution info
1.01 µg/mL
Notes

-

Tested
Tested

Species
Mouse
Dilution info
5.05 µg/mL
Notes

-

Species
Rat
Dilution info
5.05 µg/mL
Notes

-

Target data

Function

Pore-forming subunit of the rod cyclic nucleotide-gated channel. Mediates rod photoresponses at dim light converting transient changes in intracellular cGMP levels into electrical signals. In the dark, cGMP levels are high and keep the channel open enabling a steady inward current carried by Na(+) and Ca(2+) ions that leads to membrane depolarization and neurotransmitter release from synaptic terminals. Upon photon absorption cGMP levels decline leading to channel closure and membrane hyperpolarization that ultimately slows neurotransmitter release and signals the presence of light, the end point of the phototransduction cascade. Conducts cGMP- and cAMP-gated ion currents, with permeability for monovalent and divalent cations. The selectivity for Ca(2+) over Na(+) increases with cGMP concentrations, whereas the selectivity among monovalent ions is independent of the cGMP levels.

Alternative names

CNCG, CNCG1, Cyclic nucleotide-gated channel alpha-1, CNG channel alpha-1, CNG-1, CNG1, Cyclic nucleotide-gated cation channel 1, Rod photoreceptor cGMP-gated cation channel subunit alpha, cGMP-gated cation channel alpha-1

Recommended products

Mouse Recombinant Monoclonal CNGA1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
PMc 1D1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255766 is the carrier-free version of Anti-CNGA1 antibody [PMc 1D1] ab253296.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (Anti-CNGA1 antibody [PMc 1D1] ab253296).

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling CNGA1 with Anti-CNGA1 antibody [PMc 1D1] ab253296 at 1.01μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on light-sensing cells of rat retina is observed. The section was incubated with Anti-CNGA1 antibody [PMc 1D1] ab253296 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (Anti-CNGA1 antibody [PMc 1D1] ab253296).

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling CNGA1 with Anti-CNGA1 antibody [PMc 1D1] ab253296 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution. Positive staining on rat retina is observed. Nuclear counterstain is DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (Anti-CNGA1 antibody [PMc 1D1] ab253296).

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling CNGA1 with Anti-CNGA1 antibody [PMc 1D1] ab253296 at 1.01μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on light-sensing cells of mouse retina is observed. The section was incubated with Anti-CNGA1 antibody [PMc 1D1] ab253296 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (Anti-CNGA1 antibody [PMc 1D1] ab253296).

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling CNGA1 with Anti-CNGA1 antibody [PMc 1D1] ab253296 at 5.05μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution. Positive staining on mouse retina is observed. Nuclear counterstain is DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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