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AB154276

Anti-CNOT3 antibody

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(1 Publication)

Rabbit Polyclonal CNOT3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CNOT3 aa 500 to C-terminus.

View Alternative Names

KIAA0691, LENG2, NOT3, CNOT3, CCR4-NOT transcription complex subunit 3, CCR4-associated factor 3, Leukocyte receptor cluster member 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CNOT3 antibody (AB154276)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CNOT3 antibody (AB154276)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/200 dilution. Lower panel : Costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT3 antibody (AB154276)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT3 antibody (AB154276)

Immunohistochemical analysis of paraffin-embedded Human H441 xenograft, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/100 dilution.

Western blot - Anti-CNOT3 antibody (AB154276)
  • WB

Unknown

Western blot - Anti-CNOT3 antibody (AB154276)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CNOT3 antibody (ab154276) at 1/3000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CNOT3 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75175

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.
See full target information CNOT3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:4181 PubMed31519907

2019

UPF1/SMG7-dependent microRNA-mediated gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jungyun Park,Jwa-Won Seo,Narae Ahn,Seokju Park,Jungwook Hwang,Jin-Wu Nam
View all publications
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Product promise

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