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AB214937

Anti-CNOT4 antibody

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(3 Publications)

Rabbit Polyclonal CNOT4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CNOT4 aa 300-500.

View Alternative Names

NOT4, CNOT4, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, Potential transcriptional repressor NOT4Hp, RING-type E3 ubiquitin transferase CNOT4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT4 antibody (AB214937)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT4 antibody (AB214937)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling CNOT4 with ab214937 at 1/50 dilution.

Western blot - Anti-CNOT4 antibody (AB214937)
  • WB

Unknown

Western blot - Anti-CNOT4 antibody (AB214937)

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Western blot - Anti-CNOT4 antibody (ab214937) at 1/50 dilution

All lanes:

Human cell line SiHa

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CNOT4 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

O95628

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed : 11823428, PubMed : 22159038, PubMed : 26575292). Involved in activation of the JAK/STAT pathway (PubMed : 11823428, PubMed : 22159038). Catalyzes ubiquitination of methylated RBM15 (PubMed : 26575292). Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed : 29861391). As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy (PubMed : 29861391).
See full target information CNOT4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

RNA (New York, N.Y.) 30:1572-1585 PubMed39293933

2024

Translation elongation inhibitors stabilize select short-lived transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolle A Rosa-Mercado,Allen R Buskirk,Rachel Green

Molecular cell 83:2290-2302.e13 PubMed37295431

2023

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Höpfler,Eva Absmeier,Sew-Yeu Peak-Chew,Evangelia Vartholomaiou,Lori A Passmore,Ivana Gasic,Ramanujan S Hegde

Annals of translational medicine 7:723 PubMed32042739

2020

Epigenetic hypomethylation and upregulation of GD3s in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Li,Xiangjin Zheng,Liwen Ren,Weiqi Fu,Jinyi Liu,Jun Xv,Shiwei Liu,Jinhua Wang,Guanhua Du
View all publications
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Product promise

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